Department of Evidence-Based Medicine and Social Medicine, School of Public Health, Chengdu Medical College, Xindu avenue 783, Chengdu, Sichuan, 610500, China.
Department of Hepatobiliary Surgery, Qinghai Provincial Traffic Hospital, Traffic lane 7, Xining, 810001, China.
BMC Gastroenterol. 2024 Sep 30;24(1):330. doi: 10.1186/s12876-024-03422-5.
To explore the pathogenesis of different subtypes of gallstones in high-altitude populations from a molecular perspective.
We collected bile samples from 20 cholesterol gallstone disease (CGD) patients and 20 pigment gallstone disease (PGD) patients. Proteomics analysis was performed by LC/MS DIA, while metabolomics analysis was performed by UPLC- Q-TOF/MS.
We identified 154 up-regulated and 196 down-regulated differentially expressed proteins, which were significantly enriched in neurodegenerative diseases, energy metabolism, amino acid metabolism etc. In metabolomics analysis, 20 up-regulated and 63 down-regulated differentially expressed metabolites were identified, and they were significantly enriched in vitamin B6 metabolism. Three pathways of integrated proteomics and metabolomics were significantly enriched: porphyrin and chlorophyll metabolism, riboflavin metabolism and aminoacyl-tRNA biosynthesis. Remarkably, 7 differentially expressed proteins and metabolites showed excellent predictive performance and were selected as potential biomarkers.
The findings of our metabolomics and proteomics analyses help to elucidate the underlying mechanisms of gallstone formation in high-altitude populations.
从分子角度探讨高原人群不同类型胆石症的发病机制。
收集 20 例胆固醇胆石症（CGD）患者和 20 例色素胆石症（PGD）患者的胆汁样本。采用 LC/MS DIA 进行蛋白质组学分析，采用 UPLC-Q-TOF/MS 进行代谢组学分析。
共鉴定出 154 个上调和 196 个下调的差异表达蛋白，这些蛋白在神经退行性疾病、能量代谢、氨基酸代谢等方面显著富集。在代谢组学分析中，鉴定出 20 个上调和 63 个下调的差异表达代谢物，它们在维生素 B6 代谢中显著富集。整合蛋白质组学和代谢组学的三个途径显著富集：卟啉和叶绿素代谢、核黄素代谢和氨酰-tRNA 生物合成。值得注意的是，有 7 个差异表达的蛋白质和代谢物表现出优异的预测性能，被选为潜在的生物标志物。
本代谢组学和蛋白质组学分析的结果有助于阐明高原人群胆石形成的潜在机制。
