3 Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens Medical School, Athens, Greece.
Department of Medical Genetics, National and Kapodistrian University of Athens Medical School, Athens, Greece.
In Vivo. 2020 Mar-Apr;34(2):517-525. doi: 10.21873/invivo.11803.
To identify differentially expressed proteins (DEPs) in 1 trimester maternal plasma between pregnant women at risk for gestational diabetes mellitus (GDM) and uncomplicated controls.
First-trimester plasma from five women who developed GDM and five from non-diabetic ones were analyzed using isobaric tag for relative and absolute quantitation - labeled proteomics. Enzyme-linked immunosorbent assay was further applied in an independent cohort of 25 GDM cases and 25 controls for verification.
Prenylcysteine oxidase 1 (PCYOX1), beta-ala-his dipeptidase (CNDP1), extracellular matrix protein 1 (ECM1), basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2), thrombospondin-4 (TSP-4) demonstrated significant differences in expression between the two groups (p<0.05). DEPs are mainly associated with complement and coagulation cascades.
The reported plasma proteomic changes represent potential biomarkers for the early identification of women at risk for GDM. Future studies using larger and more diverse cohorts are necessary to assess the clinical utility of these findings.
鉴定妊娠糖尿病（GDM）高危孕妇和无并发症对照组孕妇 1 孕期母血浆中的差异表达蛋白（DEPs）。
采用相对和绝对定量同位素标记标签（iTRAQ）标记蛋白质组学技术分析 5 例发生 GDM 的孕妇和 5 例非糖尿病孕妇的 1 孕期血浆。进一步用酶联免疫吸附试验（ELISA）在 25 例 GDM 病例和 25 例对照组中进行验证。
在两组之间，原胱氨酸氧化酶 1（PCYOX1）、β-丙氨酸-组氨酸二肽酶（CNDP1）、细胞外基质蛋白 1（ECM1）、基底膜特异性硫酸乙酰肝素蛋白聚糖核心蛋白（HSPG2）、血小板反应蛋白 4（TSP-4）的表达存在显著差异（p<0.05）。差异表达蛋白主要与补体和凝血级联有关。
报告的血浆蛋白质组变化代表了早期识别 GDM 高危妇女的潜在生物标志物。需要使用更大和更多样化的队列进行未来研究，以评估这些发现的临床应用价值。
