Institute of Food Technology and Analysis, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, 90-537 Lodz, Poland.
Institute of Molecular and Industrial Biotechnology, Lodz University of Technology, 90-537 Lodz, Poland.
Nutrients. 2022 Jun 17;14(12):2529. doi: 10.3390/nu14122529.
The aim of the research was to use bioactive heteropolysaccharides isolated from rye bran to obtain innovative systems for the controlled release of bioactive compounds. The core of the obtained encapsulates was honey and royal jelly. It was shown for the first time that preparations effectively ameliorated inflammatory response in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, decreasing the secretion of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and nitric oxide (NO). The in vitro digestion process revealed that bee products' encapsulates were stronger oxidative stress reducers and had sustained ability to reduction in inflammation state mediators. The lack of inhibitory effect on migration rate of human microvascular endothelial cells (HMEC-1) endothelial cells and mouse embryonic fibroblasts (NIH-3T3), both cell models involved in wound healing process, additionally identified these preparations as agents potentially used in the management of inflammatory response. In the process of a simulated digestion in vitro, the innovative microcapsules showed 85% higher biostability and two to ten times better bioavailability, compared to natural bee products.
本研究旨在利用从小麦麸皮中分离出的具有生物活性的杂多糖，获得用于生物活性化合物控释的创新系统。所获得的包封物的核心是蜂蜜和蜂王浆。首次表明，这些制剂可有效改善脂多糖（LPS）处理的 RAW 264.7 巨噬细胞中的炎症反应，降低白细胞介素 6（IL-6）、肿瘤坏死因子α（TNF-α）和一氧化氮（NO）的分泌。体外消化过程表明，蜂产品包封物具有更强的抗氧化应激能力，并具有持续降低炎症状态介质的能力。缺乏对人微血管内皮细胞（HMEC-1）和小鼠胚胎成纤维细胞（NIH-3T3）的迁移率的抑制作用，这两种细胞模型均参与伤口愈合过程，此外还将这些制剂鉴定为潜在用于管理炎症反应的试剂。在体外模拟消化过程中，与天然蜂产品相比，新型微胶囊的生物稳定性高 85%，生物利用度提高了两到十倍。
