Wang Wei, Kong Xiaojun, Zhao Guangchao, Huang Xuelian, Yuan Jun, Li Na, Zhang Xiaonan, Luo Kaiyun, Luan Jianfeng, Fan Xuzhou
Department of Blood Transfusion Medicine, School of Medicine, Jinling Hospital, Nanjing University, Nanjing 210016, China.
Department of Blood Transfusion, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 211199, China.
Pathogens. 2022 Jun 20;11(6):710. doi: 10.3390/pathogens11060710.
Transfusion-transmitted infections (TTIs), such as hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), and treponema pallidum (TP), must be detected before blood transfusion. However, few studies have been conducted on the prevalence and accuracy of positive results in hospitalized patients. The purpose of this study was to investigate the real seroprevalence of TTIs among patients before blood transfusion and analyze the characteristics of false-positive results in Jinling Hospital, Nanjing University, China. TTI results were collected from medical records and analyzed retrospectively. Additionally, we also used confirmatory assays to verify the accuracy of positive results. The overall prevalence of TTI was 8.96%, which was related to gender and age. The real positive rates were 86.67% (HBV), 35.09% (HCV), 20.75% (HIV), and 100% (TP). Our results also showed that high-speed centrifugation can reduce the false-positive rate of HBsAg. In summary, the results demonstrated that the positive rates of TTIs in hospitalized patients are higher than those in the general population. We also confirmed the existence of false-positive results in serological screening for TTIs. The method of processing specimens through high-speed centrifugation could reduce the false-positive results of detecting antigens effectively.
输血传播感染（TTIs），如乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）、人类免疫缺陷病毒（HIV）和梅毒螺旋体（TP），必须在输血前进行检测。然而，针对住院患者阳性结果的患病率和准确性的研究较少。本研究旨在调查中国南京大学金陵医院输血前患者中TTIs的实际血清学患病率，并分析假阳性结果的特征。从病历中收集TTI结果并进行回顾性分析。此外，我们还使用确证试验来验证阳性结果的准确性。TTI的总体患病率为8.96%，与性别和年龄有关。实际阳性率分别为86.67%（HBV）、35.09%（HCV）、20.75%（HIV）和100%（TP）。我们的结果还表明，高速离心可以降低HBsAg的假阳性率。总之，结果表明住院患者中TTIs的阳性率高于普通人群。我们还证实了TTIs血清学筛查中存在假阳性结果。通过高速离心处理标本的方法可以有效降低检测抗原的假阳性结果。
