Veiga Alan A, Irioda Ana Carolina, Mogharbel Bassam F, Bonatto Sandro J R, Souza Lauro M
Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80250-060, Brazil.
Faculdades Pequeno Príncipe, Curitiba 80230-020, Brazil.
Pharmaceuticals (Basel). 2022 Jun 16;15(6):754. doi: 10.3390/ph15060754.
Adrenocortical carcinoma (ACC) is a rare subtype of cancer, with a poor prognosis in children and adults. Mitotane is the only approved adrenolytic drug for the treatment of ACC, which has controversies regarding its efficacy and side effects on patients. Onion (), a worldwide consumed food, is associated with many health benefits. Along with its glycosides, the flavonoid quercetin is abundant in onions. After evaluating the cytotoxicity of extracts on adrenocortical carcinoma cell line (H295R), the rich quercetin fractions had better results. Then, we aimed to compare the quercetin vs. mitotane effectiveness, using adrenocortical carcinoma cell lines H295R and SW-13. Quercetin showed a higher cytotoxicity response on both cancerous cell lines after 10 µM concentration, while mitotane only after 30 µM. Cell cycle dynamics were altered upon quercetin treatments, with G2 phase increase with 30 µM of quercetin on H295R cell line and G1 arrest on SW-13 cell line with 15 µM. Early and late apoptosis, alongside intracellular calcium, were increased on SW-13 treated with 30 µM of quercetin, and ROS rates were reduced by quercetin on H295R. Therefore, quercetin-rich onions have the potential to be a natural source of anticancer agents for adrenocortical carcinoma.
肾上腺皮质癌（ACC）是一种罕见的癌症亚型，在儿童和成人中预后较差。米托坦是唯一被批准用于治疗ACC的肾上腺溶解药物，但其对患者的疗效和副作用存在争议。洋葱是一种全球广泛食用的食物，具有许多健康益处。除了其糖苷外，洋葱中富含类黄酮槲皮素。在评估提取物对肾上腺皮质癌细胞系（H295R）的细胞毒性后，富含槲皮素的组分效果更好。然后，我们旨在使用肾上腺皮质癌细胞系H295R和SW-13比较槲皮素与米托坦的有效性。在10μM浓度下，槲皮素对两种癌细胞系均表现出更高的细胞毒性反应，而米托坦仅在30μM时才有此反应。槲皮素处理后细胞周期动力学发生改变，在H295R细胞系中30μM槲皮素使G2期增加，在SW-13细胞系中15μM槲皮素使G1期停滞。用30μM槲皮素处理的SW-13细胞早期和晚期凋亡以及细胞内钙增加，槲皮素使H295R细胞系中的活性氧率降低。因此，富含槲皮素的洋葱有可能成为肾上腺皮质癌抗癌剂的天然来源。
