Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Mochida Pharmaceutical Co. Ltd., 1-1 Ichigaya honmuracho, Shinjuku-ku, Tokyo 162-0845, Japan.
Biomater Adv. 2022 Jun;137:212825. doi: 10.1016/j.bioadv.2022.212825. Epub 2022 Apr 30.
In surgery, both antiperitoneal adhesion barriers and hemostats with high efficiency and excellent handling are necessary. However, antiadhesion and hemostasis have been examined separately. In this study, six different ultrapure alginate bilayer sponges with thicknesses of 10, 50, 100, 200, 300, and 500 μm were fabricated via lyophilization and subsequent mechanical compression. Compression significantly enhanced mechanical strength and improved handling. Furthermore, it had a complex effect on dissolution time and contact angle. Therefore, the 100 μm compressed sponge showed the highest hemostatic activity in the liver bleeding model in mice, whereas the 200 μm sponge demonstrated the highest antiadhesion efficacy among the compressed sponges in a Pean crush hepatectomy-induced adhesion model in rats. For the first time, we systematically evaluated the effect of sponge compression on foldability, fluid absorption, mechanical strength, hemostatic effect, and antiadhesion properties. The optimum thickness of an alginate bilayer sponge by compression balances antiperitoneal adhesion and hemostasis simultaneously.
在外科手术中，既需要具有高效能和良好操作性的防腹膜粘连屏障，也需要高效能和良好操作性的止血剂。然而，人们已经分别对防粘连和止血性能进行了研究。在本研究中，我们通过冻干法和后续的机械压缩，制备了 6 种不同厚度（10、50、100、200、300 和 500μm）的双层超纯海藻酸钠海绵。压缩显著增强了机械强度并改善了操作性能。此外，它对溶解时间和接触角有复杂的影响。因此，在小鼠肝脏出血模型中，100μm 压缩海绵表现出最高的止血活性，而在大鼠的 Pean 挤压肝切除术诱导粘连模型中，200μm 海绵在压缩海绵中表现出最高的防粘连效果。我们首次系统地评估了海绵压缩对折叠性、吸水性、机械强度、止血效果和防粘连性能的影响。通过压缩得到的最佳厚度的海藻酸钠双层海绵可以同时平衡防腹膜粘连和止血性能。
