Seo Kwon Ho, Lee Kyung Eun, Yanilmaz Meltem, Kim Juran
Advanced Textile R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan 15588, Korea.
Department of Mechanical Engineering, Inha University, 100 Inharo, Incheon 22212, Korea.
Pharmaceutics. 2022 Jun 15;14(6):1272. doi: 10.3390/pharmaceutics14061272.
In this study, we aimed to explore the morphologies of porous poly(lactic acid) (PLA) fibers through liquid−liquid phase separation, and investigate the relationship among pore formation, physical properties, and antibacterial activities of the fibers for identifying their potential as drug delivery carriers. Antibacterial activities of gentamicin-, kanamycin-, and amikacin-loaded PLA fibers against E. coli and S. epidermidis were evaluated. The antibacterial activity of drugs against E. coli showed the following profile: gentamicin > amikacin > kanamycin; however, S. epidermidis growth was almost completely inhibited immediately after the administration of all three drugs. The efficiency of gentamicin can be attributed to the electrostatic interactions between the positively and negatively charged antibiotic and bacterial cell membrane, respectively. Furthermore, gentamicin-loaded porous PLA fibers were evaluated as drug delivery systems. The cumulative amount of gentamicin in porous PLA nanofibers was considerably higher than that in other PLA fibers for 168 h, followed by 7:3 PLA > 6:4 PLA > 5:5 PLA > non-porous PLA. The 7:3 PLA fibers were projected to be ideal drug carrier candidates for controlled antibiotic release in delivery systems owing to their interconnected internal structure and the largest surface area (55.61 m2 g−1), pore size (42.19 nm), and pore volume (12.78 cm3 g−1).
在本研究中，我们旨在通过液-液相分离探索多孔聚乳酸（PLA）纤维的形态，并研究纤维的孔隙形成、物理性质和抗菌活性之间的关系，以确定其作为药物递送载体的潜力。评估了负载庆大霉素、卡那霉素和阿米卡星的PLA纤维对大肠杆菌和表皮葡萄球菌的抗菌活性。药物对大肠杆菌的抗菌活性表现出以下特征：庆大霉素>阿米卡星>卡那霉素；然而，在施用所有三种药物后，表皮葡萄球菌的生长几乎立即被完全抑制。庆大霉素的有效性可归因于带正电荷和负电荷的抗生素与细菌细胞膜之间的静电相互作用。此外，对负载庆大霉素的多孔PLA纤维作为药物递送系统进行了评估。在168小时内，多孔PLA纳米纤维中庆大霉素的累积量显著高于其他PLA纤维，其次是7:3 PLA>6:4 PLA>5:5 PLA>无孔PLA。由于其相互连接的内部结构以及最大的表面积（55.61 m2 g−1）、孔径（42.19 nm）和孔体积（12.78 cm3 g−1），7:3 PLA纤维被认为是递送系统中控制抗生素释放的理想药物载体候选物。
