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纳米载体用于癌症化疗靶向治疗的机制研究：新型药物递送系统的新策略

Mechanistic Approach of Nano Carriers for Targeted in Cancer Chemotherapy: A Newer Strategy for Novel Drug Delivery System.

作者信息

Dey Niladri Shekhar

机构信息

Jiaganj Institute of Pharmacy, Jiaganj, Murshidabad 742123, India.

出版信息

Polymers (Basel). 2022 Jun 8;14(12):2321. doi: 10.3390/polym14122321.

DOI:10.3390/polym14122321
PMID:35745897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9231136/
Abstract

The application of nanomedicine represents an innovative approach for the treatment in the modern field of cancer chemotherapy. In the present research work, tamoxifen citrate loaded nanolipid vesicles were prepared conjugated with phosphoethanolamine as the linker molecule, and the specific antibody was tagged with the linker molecule on the bilayer surface of the vesicles. The main objective of this study is to determine the efficacy and biological behavior of antibody conjugated nanoliposome in breast cancer cell lines. Percentage of drug loading and loading efficiency was done and their results were compared to theoretical drug loading. The average diameter of those vesicles was within the 100 nm range, which is revealed in FESEM and TEM images and their lamellarity was observed in cryo-TEM images. The hydrodynamic diameter was done by particle size analysis and the surface charge was determined by the zeta potential parameter. Predominant cellular uptake was observed for antibody conjugated nanolipid vesicles in MCF-7 and MDA-MB-453 human breast cancer cell lines. A cellular apoptosis assay was conducted by flow cytometer (FACS). All experimental data would be more beneficial for the treatment of breast cancer chemotherapy. Further studies are warranted to investigate the efficacy and safety of antibody conjugated nanolipid vesicles in vivo for breast cancer animal model.

摘要

纳米医学的应用代表了现代癌症化疗领域中一种创新的治疗方法。在本研究工作中，制备了负载柠檬酸他莫昔芬的纳米脂质体，并与磷酸乙醇胺作为连接分子进行共轭，且在囊泡的双层表面用连接分子标记了特异性抗体。本研究的主要目的是确定抗体共轭纳米脂质体在乳腺癌细胞系中的疗效和生物学行为。测定了载药百分比和载药效率，并将其结果与理论载药量进行比较。这些囊泡的平均直径在100 nm范围内，这在场发射扫描电子显微镜（FESEM）和透射电子显微镜（TEM）图像中得以显示，并且在冷冻透射电子显微镜（cryo-TEM）图像中观察到了它们的片层结构。通过粒度分析测定了流体动力学直径，并通过zeta电位参数确定了表面电荷。在MCF-7和MDA-MB-453人乳腺癌细胞系中观察到抗体共轭纳米脂质体具有主要的细胞摄取。通过流式细胞仪（FACS）进行了细胞凋亡测定。所有实验数据对乳腺癌化疗的治疗将更有益。有必要进一步研究抗体共轭纳米脂质体在乳腺癌动物模型体内的疗效和安全性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/732a372c45d6/polymers-14-02321-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/856735f77db6/polymers-14-02321-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/260dabc3d235/polymers-14-02321-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/d9b343093588/polymers-14-02321-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/9d5972c83f7a/polymers-14-02321-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/bae51e87696e/polymers-14-02321-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/0297c24713db/polymers-14-02321-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/658e290d0252/polymers-14-02321-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/732a372c45d6/polymers-14-02321-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/4e6677450719/polymers-14-02321-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/2a8dbd02f8e7/polymers-14-02321-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/0f984bf2efa2/polymers-14-02321-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/856735f77db6/polymers-14-02321-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/260dabc3d235/polymers-14-02321-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/d9b343093588/polymers-14-02321-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/9d5972c83f7a/polymers-14-02321-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/bae51e87696e/polymers-14-02321-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/0297c24713db/polymers-14-02321-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/658e290d0252/polymers-14-02321-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d848/9231136/732a372c45d6/polymers-14-02321-g011.jpg

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