Nademi Azam, Shams-Ghahfarokhi Masoomeh, Razzaghi-Abyaneh Mehdi
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Mycology, Pasteur Institute of Iran, Tehran, Iran.
Curr Med Mycol. 2021 Dec;7(4):28-33. doi: 10.18502/cmm.7.4.8408.
This study aimed to assess the effect of ethanolic extract (ACE) loaded polyacrylonitrile (PAN) and polyvinyl pyrrolidone (PVP) nanofibers on () and genes expression.
The minimum inhibitory concentrations (MICs) of ACE against ATCC 10231 and clinical fluconazole (FLC)-resistant PFCC 93-902 were determined using the Clinical and Laboratory Standards Institute (CLSI) protocol (M27-Ed4) at a concentration range of 45.3-5800 µg/ml. The nanofibers containing ACE (60 wt%) were fabricated using the electrospinning technique. The expression of the and genes was studied in the fungus exposed to ACE-loaded nanofibers and 0.5×MIC concentration of FLC using the real-time polymerase chain reaction.
MIC and MIC of ACE against FLC-resistant were 725 and 1450 μg/mL, respectively. The expression of (4.5-fold) and (6.3-fold) were down-regulated after the exposure of FLC-resistant to ACE-loaded nanofibers (<0.05). Furthermore, the expression of (2.8-fold) and (3.2-fold) were up-regulated in FLC-treated (<0.05).
The results revealed that nanofibers containing ACE interact with drug-resistant genes expressed in . Further studies are recommended to investigate the mode of action and other biological activities of ACE-loaded nanofibers against and other pathogenic fungi.
本研究旨在评估负载乙醇提取物（ACE）的聚丙烯腈（PAN）和聚乙烯吡咯烷酮（PVP）纳米纤维对（）和基因表达的影响。
采用美国临床和实验室标准协会（CLSI）的方案（M27 - Ed4），在45.3 - 5800μg/ml的浓度范围内测定ACE对白色念珠菌ATCC 10231和临床氟康唑（FLC）耐药的近平滑念珠菌PFCC 93 - 902的最低抑菌浓度（MIC）。使用静电纺丝技术制备含60 wt% ACE的纳米纤维。采用实时聚合酶链反应研究暴露于负载ACE纳米纤维和0.5×MIC浓度氟康唑的真菌中（）和基因的表达。
ACE对氟康唑耐药近平滑念珠菌的MIC和MIC分别为725和1450μg/mL。氟康唑耐药近平滑念珠菌暴露于负载ACE纳米纤维后，（）（4.5倍）和（）（6.3倍）的表达下调（<0.05）。此外，在氟康唑处理的近平滑念珠菌中，（）（2.8倍）和（）（3.2倍）的表达上调（<0.05）。
结果表明，含ACE的纳米纤维与近平滑念珠菌中表达的耐药基因相互作用。建议进一步研究以探讨负载ACE纳米纤维对近平滑念珠菌和其他致病真菌的作用方式及其他生物学活性。
