Characterization of species isolated from vulvovaginal candidiasis by MALDI-TOF with antifungal susceptibility profiles.

BACKGROUND AND PURPOSE

Vulvovaginal candidiasis (VVC) is an opportunistic infection due to species, one of the most common genital tract diseases among reproductive-age women. The present study aimed to investigate the prevalence of VVC among non-pregnant women and identify the epidemiology of the involved species with the evaluation of antifungal susceptibilities.

MATERIALS AND METHODS

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed to identify species isolated from the genital tract of 350 non-pregnant women. Moreover, antifungal susceptibility testing was performed according to the Clinical and Laboratory Standards Institute broth microdilution method guidelines (M27-A3 and M27-S4).

RESULTS

Vaginal swab cultures of 119 (34%) women yielded species. was the most frequently isolated species (68%), followed by (19.2%). Voriconazole was the most active drug against all tested isolates showing an MIC50/MIC90 corresponding to 0.016/0.25 µg/mL, followed by posaconazole (0.031/1 µg/mL). Overall, resistance rates to fluconazole, itraconazole, and voriconazole were 2.4%, 4.8% and, 0.8% respectively. However, posaconazole showed potent activity against all tested isolates.

CONCLUSION

Results of the current study showed that for the effectual therapeutic outcome of candidiasis, accurate identification of species, appropriate source control, suitable antifungal regimens, and improved antifungal stewardship are highly recommended for the management and treatment of infection with , like VVC.