Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, P.O. Box: 14155-6453, Tehran, Iran.
BMC Res Notes. 2021 Mar 10;14(1):93. doi: 10.1186/s13104-021-05498-8.
The objectives of this study were to investigate the antifungal susceptibility and genetic diversity of Candida albicans isolated from HIV patients with oropharyngeal candidiasis. A total of 50 C. albicans isolates were cultured on Sabouraud glucose agar containing chloramophenicol. The antifungal susceptibility of the isolates against fluconazole, clotrimazole, nystatin, amphotericin B, ketoconazole and flucytosine was assessed using disc diffusion method. The genetic diversity of C. albicans isolates was determined using random amplified polymorphic DNA marker.
The inhibition zones ranged from 4 ± 1.8 to 40 ± 3.8 mm for fluconazole, 7 ± 1.0 to 37 ± 1.8 mm for ketoconazole, 14 ± 0.8 to24 ± 0.8 mm for amphotericin B, 25 ± 0.0 to 33 ± 0.0 mm for nystatin and 7 ± 4.2 to 40 ± 0.0 mm for clotrimazole. At 90% similarity, three distinct groups were observed. The smallest cluster composed of 3 isolates, whereas the largest one composed of 17 isolates. 32% (16/50), 28% (14/50) and 14% (7/50) were resistant to fluconazole, ketoconazole and clotrimazole, respectively.
本研究旨在调查分离自 HIV 合并口咽念珠菌病患者的白色念珠菌的抗真菌药敏性和遗传多样性。共培养 50 株白色念珠菌在含有氯苯吩嗪的沙氏葡萄糖琼脂上。采用纸片扩散法检测分离株对氟康唑、克霉唑、制霉菌素、两性霉素 B、酮康唑和氟胞嘧啶的抗真菌敏感性。采用随机扩增多态性 DNA 标记法检测白色念珠菌分离株的遗传多样性。
氟康唑的抑菌圈直径为 4±1.8 至 40±3.8mm，酮康唑为 7±1.0 至 37±1.8mm，两性霉素 B 为 14±0.8 至 24±0.8mm，制霉菌素为 25±0.0 至 33±0.0mm，克霉唑为 7±4.2 至 40±0.0mm。在 90%相似性水平下，观察到三个不同的组。最小的簇由 3 株分离株组成，而最大的簇由 17 株分离株组成。32%（16/50）、28%（14/50）和 14%（7/50）的分离株分别对氟康唑、酮康唑和克霉唑耐药。
