早期生活忽视大鼠模型中前额皮质内侧神经元兴奋性的改变。
Distinct neuronal excitability alterations of medial prefrontal cortex in early-life neglect model of rats.
机构信息
NHC Key Laboratory of Human Disease Comparative Medicine, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS); Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, China.
National Human Diseases Animal Model Resource Center, Beijing, China.
出版信息
Animal Model Exp Med. 2022 Jun;5(3):274-280. doi: 10.1002/ame2.12252. Epub 2022 Jun 23.
OBJECT
Early-life neglect has irreversible emotional effects on the central nervous system. In this work, we aimed to elucidate distinct functional neural changes in medial prefrontal cortex (mPFC) of model rats.
METHODS
Maternal separation with early weaning was used as a rat model of early-life neglect. The excitation of glutamatergic and GABAergic neurons in rat mPFC was recorded and analyzed by whole-cell patch clamp.
RESULTS
Glutamatergic and GABAergic neurons of mPFC were distinguished by typical electrophysiological properties. The excitation of mPFC glutamatergic neurons was significantly increased in male groups, while the excitation of mPFC GABAergic neurons was significant in both female and male groups, but mainly in terms of rest membrane potential and amplitude, respectively.
CONCLUSIONS
Glutamatergic and GABAergic neurons in medial prefrontal cortex showed different excitability changes in a rat model of early-life neglect, which can contribute to distinct mechanisms for emotional and cognitive manifestations.
目的
早期生活忽视对中枢神经系统具有不可逆转的情绪影响。在这项工作中，我们旨在阐明模型大鼠内侧前额叶皮质（mPFC）的不同功能神经变化。
方法
采用早期断奶的母婴分离作为大鼠早期生活忽视模型。通过全细胞膜片钳记录和分析大鼠 mPFC 中谷氨酸能和 GABA 能神经元的兴奋。
结果
mPFC 的谷氨酸能和 GABA 能神经元通过典型的电生理特性来区分。雄性组中 mPFC 谷氨酸能神经元的兴奋明显增加，而雌性和雄性组中 mPFC GABA 能神经元的兴奋均显著增加，但主要是在静息膜电位和振幅方面。
结论
在早期生活忽视的大鼠模型中，内侧前额叶皮质的谷氨酸能和 GABA 能神经元表现出不同的兴奋性变化，这有助于情绪和认知表现的不同机制。
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