Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, Salerno, Italy.
Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, I-86090, Pesche, Isernia, Italy.
J Nat Prod. 2022 Jul 22;85(7):1667-1680. doi: 10.1021/acs.jnatprod.1c01093. Epub 2022 Jun 24.
Sixteen diterpenes (-), along with 10 previously described compounds, including four flavonoids and six diterpenes, were isolated from the aerial parts of growing in Saudi Arabia. The diterpene structures were elucidated using NMR spectroscopy and mass spectrometry data. Furthermore, a DFT/NMR procedure was used to suggest the relative configuration of several compounds. The labdane-derived skeletons, namely, -atisane, -beyerene, -trachylobane, and -kaurene, were identified. The extracts, fractions, and pure compounds were then tested against , , , and . One diterpenoid, namely, psiadin, showed an additive effect with the antiseptic chlorhexidine, with a fractional inhibitory concentration index of less than 1. Additionally, psiadin showed a prospective inhibition activity for bacterial efflux pumps.
从生长在沙特阿拉伯的地上部分中分离得到了十六种二萜（-），以及十种先前描述的化合物，包括四种类黄酮和六种二萜。通过 NMR 光谱和质谱数据阐明了二萜的结构。此外，还使用 DFT/NMR 程序来提出几种化合物的相对构型。鉴定出了源自劳丹烷的骨架，即 -atisane、-beyerene、-trachylobane 和 -kaurene。然后对提取物、馏分和纯化合物进行了测试，以对抗金黄色葡萄球菌、表皮葡萄球菌、铜绿假单胞菌和白色念珠菌。一种二萜化合物，即 psiadin，与防腐剂洗必泰表现出相加作用，其部分抑菌浓度指数小于 1。此外，psiadin 显示出对细菌外排泵的潜在抑制活性。
