Isolation and characterisation of pharmaceutically versatile molecules from and evaluation of their cytotoxic activity against human cancer cell lines.

The disclosed study reveals isolation, characterization and anticancer evaluation of . The extracts and isolated compounds were used for cytotoxic activity against (lung (A549), pancreatic (MIAPaCa), colon (HCT-116), breast (MDA-MB-231) and breast (MDA-MB-468) cell lines. The extracts were screened for cytotoxicity using MTT colorimetric assay. Out of all extracts, methanolic (30) %: chloroform fraction (TAW6) with 75.01% inhibition at a concentration 100 µg/mL was observed. The selected extracts were further processed for column chromatography and led to isolation of seven compounds (A to G). The structural determination of isolated compounds was carried out using HNMR, CNMR, IR and HRMS. All the isolates were tested for cytotoxic activity and compound B was found most active with IC values 11.29 µg against HCT-116 (Colon). The compound B was then used for detailed study via and . Thus the significant anticancer activity particularly against colon cancerous cell lines recommends that the ( could act as a potential drug candidate for cancer, more particularly for colon cancer.