Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Jl. Raya Bogor, Km. 46, Cibinong, Bogor Regency 16911, Indonesia.
Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Jl. Raya Bogor, Km. 46, Cibinong, Bogor Regency 16911, Indonesia; School of Chemistry and Molecular Bioscience, University of Queensland, Chemistry Bld, 68 Cooper Rd, Brisbane, QLD 4072, Australia.
Int J Biol Macromol. 2022 Aug 1;214:426-438. doi: 10.1016/j.ijbiomac.2022.06.117. Epub 2022 Jun 21.
A novel D-allulose 3-epimerase (DAEase) from Arthrobacter psychrolactophilus (Ap DAEase) was first characterized in this study. The enzyme catalyzes the epimerization of d-fructose into a functional rare sugar, D-allulose. Ap DAEase was the first record of DAEase identified as a homotrimer with the molecular weight of its subunit at approximately 34 kDa. It had an optimum activity at pH 8.5 and 70 °C in the presence of 1 mM Mg. Ap DAEase was found to be an excellent thermostable enzyme. The half-life value at 70 °C was 128.4 min. The k and catalytic efficiency of the enzyme toward d-fructose were 2920.00 s and 3.953 mM s, respectively. To the best of our knowledge, Ap DAEase possesses the highest k among the previously reported DAEases. The conversion ratio of 500 and 100 mg Ld-fructose to D-allulose was approximately 27 % in 15 and 90 min, respectively. These research findings suggest that Ap DAEase is a promising candidate for the industrial production of D-allulose.
本研究首次对来自嗜冷节杆菌（Arthrobacter psychrolactophilus）的新型 D-阿洛酮糖 3-差向异构酶（DAEase）进行了表征。该酶催化 D-果糖差向异构化为具有功能的稀有糖 D-阿洛酮糖。Ap DAEase 是首个被鉴定为三聚体的 DAEase，其亚基分子量约为 34 kDa。在 pH 8.5 和 70°C 下，1 mM Mg 存在时，该酶具有最佳活性。Ap DAEase 是一种出色的耐热酶。在 70°C 下的半衰期值为 128.4 分钟。该酶对 D-果糖的 k 和催化效率分别为 2920.00 s 和 3.953 mM s。据我们所知，Ap DAEase 具有先前报道的 DAEase 中最高的 k 值。在 15 和 90 分钟内，将 500 和 100 mg L D-果糖转化为 D-阿洛酮糖的转化率分别约为 27%。这些研究结果表明，Ap DAEase 是工业生产 D-阿洛酮糖的有前途的候选者。
