Department of Chemistry, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, 131039, Haryana, India.
Department of Biomedical Engineering, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, 131039, Haryana, India.
Environ Sci Pollut Res Int. 2023 Sep;30(44):98563-98580. doi: 10.1007/s11356-022-21558-3. Epub 2022 Jun 24.
An amperometric non-enzymatic glucose sensing based on polyvinylpyrrolidone and titanium dioxide nanocomposites (PVP-TiO NCs) fabricated over stainless-steel (SS) electrode was experimented. The electrode was fabricated of PVP-TiO NCs onto SS surface through drop casting coating. The NCs and the electrode were analyzed using Fourier transform infrared (FTIR) and UV-visible (UV-Vis) spectroscopy, X-ray diffraction spectra (XRD), X-ray photoelectron spectra (XPS), particle analyzer, scanning electron microscopy (SEM), EDS and transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET) analysis for surface. For electrochemical investigation, cyclic voltammetry, electrochemical impedance spectra, including Nyquist plots and Bode plots, and chronoamperometry were adopted. PVP-TiO/SS-modified electrode manifested high sensitivity of 360.13 µA/mM.cm, lower detection limit of 756.8 µM and 0 to 13 mM linear range with regression coefficient of R = 0.992. The electrode exhibited high stability with good anti-interference tendency against fructose, uric acid, ascorbic acid and sucrose. The modified electrode also performed well in real sample glucose detection. Further, PVP-TiO NCs performed appreciably toward antibacterial studies against some Gram-positive and Gram-negative deleterious bacteria, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Shigella flexneri microbes.
基于聚维酮和二氧化钛纳米复合材料（PVP-TiO NCs）的安培非酶葡萄糖传感在不锈钢（SS）电极上进行了实验。该电极是通过滴铸涂层在 SS 表面上制造的 PVP-TiO NCs。通过傅里叶变换红外（FTIR）和紫外可见（UV-Vis）光谱、X 射线衍射光谱（XRD）、X 射线光电子能谱（XPS）、颗粒分析仪、扫描电子显微镜（SEM）、能谱仪（EDS）和透射电子显微镜（TEM）以及比表面积分析（BET）对 NCs 和电极进行了分析。为了进行电化学研究，采用了循环伏安法、电化学阻抗谱，包括奈奎斯特图和波特图以及计时安培法。PVP-TiO/SS 修饰电极表现出 360.13 µA/mM.cm 的高灵敏度、756.8 µM 的低检测限和 0 至 13 mM 的线性范围，回归系数 R=0.992。该电极具有高稳定性和良好的抗干扰倾向，对果糖、尿酸、抗坏血酸和蔗糖具有良好的抗干扰倾向。修饰电极在实际样品葡萄糖检测中也表现良好。此外，PVP-TiO NCs 对一些革兰氏阳性和革兰氏阴性有害细菌，如大肠杆菌、肺炎克雷伯菌、金黄色葡萄球菌、福氏志贺菌微生物，表现出明显的抗菌研究作用。
