Litts J C, Colwell G W, Chakerian R L, Quatrano R S
Nucleic Acids Res. 1987 Apr 24;15(8):3607-18. doi: 10.1093/nar/15.8.3607.
The nucleotide sequence of a cDNA clone isolated from developing wheat embryos and encoding the Em protein is reported. The entire coding region for Em and the 3' non-translated flank are contained within this clone. The amino acid sequence deduced for Em is very rich in glycine (18 mol%) as well as both basic and acidic residues. The molecular weight of the protein is ca. 9,900 daltons. The deduced sequence is supported by direct amino acid sequencing of cyanogen bromide cleavage fragments obtained from purified Em protein. Em is shown by Southern blots to be a product of a gene family of approximately ten members.
报道了从发育中的小麦胚中分离出的一个编码Em蛋白的cDNA克隆的核苷酸序列。该克隆包含Em的整个编码区和3'非翻译侧翼区。推导的Em氨基酸序列富含甘氨酸（18摩尔%）以及碱性和酸性残基。该蛋白的分子量约为9900道尔顿。推导的序列得到了从纯化的Em蛋白获得的溴化氰裂解片段直接氨基酸测序的支持。Southern杂交显示Em是一个约有十个成员的基因家族的产物。
