Guiltinan M J, Niu X
Department of Horticulture, Pennsylvania State University, University Park 16802, USA.
Plant Mol Biol. 1996 Mar;30(6):1301-6. doi: 10.1007/BF00019560.
A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings, whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
从小麦中分离出了一个编码富含甘氨酸的RNA结合蛋白whGRP-1的cDNA。WhGRP-1包含两个保守结构域,即RNA结合基序(RNP基序)与一系列富含甘氨酸的不完全重复序列相结合,这是植物RNA结合蛋白保守家族的特征。Northern分析表明,whGRP-1 mRNA在小麦幼苗的根中积累到高水平,而在叶中积累到较低水平,幼苗中外源脱落酸不会增强whGRP-1 mRNA的积累,并且在小麦胚胎发育过程中积累到非常高的水平,显示出与ABA诱导的小麦Em基因不同的模式。