Curry J, Morris C F, Walker-Simmons M K
U.S. Department of Agriculture, Washington State University, Pullman 99164-6420.
Plant Mol Biol. 1991 Jun;16(6):1073-6. doi: 10.1007/BF00016078.
A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475-486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23,000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.
已从小麦中对第3组胚胎后期丰富（LEA）蛋白的一个cDNA克隆（pMA2005）进行了测序。该小麦cDNA克隆编码一种具有11个氨基酸序列的十个串联重复的蛋白质，并且与在大麦、胡萝卜、棉花和油菜中报道的其他第3组LEA蛋白具有同源性（L. 杜尔等人，《植物分子生物学》12: 475 - 486，1989）。推导的氨基酸序列表明该小麦蛋白的分子量为23000，是一种碱性亲水性蛋白。用该cDNA克隆进行的Northern分析表明，小麦茎组织的脱水导致转录本水平升高，这与内源脱落酸的增加相关。
