比较严重婴幼儿龋和无龋儿童巨细胞病毒的实时定量 PCR 检测。
Comparative real-time PCR quantification of cytomegalovirus in severe early childhood caries and caries-free children.
机构信息
Department of Pediatric Dentistry, Torabinejad Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pediatric Dentistry, Mazandaran University of Medical Sciences, Mazandaran, Iran.
出版信息
Eur Arch Paediatr Dent. 2022 Oct;23(5):797-801. doi: 10.1007/s40368-022-00711-z. Epub 2022 Jun 25.
PURPOSE
The present study was undertaken to evaluate cytomegalovirus (CMV) load in carious lesions in children with severe early childhood caries (S-ECC) and compare it with caries-free (CF), healthy children, by quantitative real-time polymerase chain reaction (PCR).
MATERIALS AND METHODS
Dental plaque samples were collected from 57 subjects including S-ECC (n = 33) and CF (n = 24) patients. The two groups were matched in terms of age, gender, and socioeconomic status. After deoxyribonucleic acid (DNA) extraction, SYBR Green real-time PCR was used to quantify CMV DNA.
RESULTS
The CMV DNA was detected in seven patients in the S-ECC group. The mean of decayed, missing, and filled tooth surfaces (dmfs) was 11.90 ± 5.95 in the S-ECC group. There was no significant difference in gender between the two groups. There was a significant relationship between CMV positivity in children and the S-ECC group (p = 0.043).
CONCLUSION
Results of this study suggest a relationship between the presence of CMV in the oral cavity and S-ECC. We can design clinical trials to confirm these results and improve children's dental care.
目的
本研究通过实时定量聚合酶链反应（PCR）评估严重婴幼儿龋（S-ECC）患儿龋损部位的巨细胞病毒（CMV）载量，并与无龋（CF）、健康儿童进行比较。
材料和方法
采集 57 名受试者（包括 S-ECC [n=33]和 CF [n=24]患者）的牙菌斑样本。两组在年龄、性别和社会经济地位方面相匹配。提取脱氧核糖核酸（DNA）后，采用 SYBR Green 实时 PCR 定量 CMV DNA。
结果
S-ECC 组中 7 名患者检测到 CMV DNA。S-ECC 组的龋失补牙面数（dmfs）平均值为 11.90±5.95。两组的性别无显著差异。CMV 阳性与 S-ECC 组之间存在显著关系（p=0.043）。
结论
本研究结果表明口腔中 CMV 的存在与 S-ECC 之间存在关系。我们可以设计临床试验来证实这些结果并改善儿童的口腔护理。
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