Pharmacy College, Henan University of Chinese Medicine, Zhengzhou 450046, People's Republic of China.
Pharmacy College, Henan University of Chinese Medicine, Zhengzhou 450046, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Nov 5;280:121535. doi: 10.1016/j.saa.2022.121535. Epub 2022 Jun 20.
In this work, a novel fluorescent biosensor for sensitive detecting of aflatoxin B (AFB) was constructed through activators regenerated by electron transfer for atom transfer radical polymerization (ARGET-ATRP) for the first time. The AFB antigen was immobilized on the carboxy magnetic beads (MBs) by forming a sandwich-type "aptamer-antigen-antibody" immune system. Then, acrylamid (AM) was introduced through ARGET-ATRP to provide binding sites for the signaling molecules. Finally, carboxy porphyrins (TPP*) were connected with monomers through an amide bond and fixed on the MBs. Under the optimal experimental conditions, the fluorescence intensity and the logarithm of the concentration of AFB showed a good relationship from 100 fg mL to 100 ng mL, with the limit of detection (LOD) as low as 8.38 fg mL. In addition, the method shows good selectivity and excellent reproducibility. More importantly, the biosensor has applied to the quantitative analysis of AFB in four Chinese medicines, and this strategy could potentially serve as a novel means for sensitive detecting of AFB in complex matrices.
在这项工作中，通过电子转移供体再生原子转移自由基聚合（ARGET-ATRP），首次构建了用于灵敏检测黄曲霉毒素 B（AFB）的新型荧光生物传感器。AFB 抗原通过形成三明治型“适体-抗原-抗体”免疫体系固定在羧基磁性珠（MBs）上。然后，通过 ARGET-ATRP 引入丙烯酰胺（AM），为信号分子提供结合位点。最后，通过酰胺键将羧基卟啉（TPP*）与单体连接并固定在 MBs 上。在最佳实验条件下，荧光强度与 AFB 浓度的对数呈良好的线性关系，其检测限（LOD）低至 8.38 fg mL。此外，该方法具有良好的选择性和优异的重现性。更重要的是，该生物传感器已应用于四种中药中 AFB 的定量分析，该策略有望成为复杂基质中灵敏检测 AFB 的新方法。
