基于 CRISPR/Cas12a 整合 MXenes 的新型荧光生物传感器用于检测黄曲霉毒素 B1。
A novel fluorescence biosensor based on CRISPR/Cas12a integrated MXenes for detecting Aflatoxin B1.
机构信息
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Food Refrigeration and Computerized Food Technology (FRCFT), Agriculture and Food Science Centre, University College Dublin, National University of Ireland, Belfield, Dublin 4, Ireland.
出版信息
Talanta. 2023 Jan 15;252:123773. doi: 10.1016/j.talanta.2022.123773. Epub 2022 Aug 6.
Aflatoxin B1 (AFB1) contamination in food threatens global food safety, and rapid quantitative detection of AFB1 remains a challenge. Herein, a novel fluorescence biosensor was developed for AFB1 detection based on CRISPR/Cas12a and MXenes. Specifically, the well-designed activator was locked by dual-AFB1 aptamers, Cas12a was directly linked to crRNA to form inactivated complexes, and MXenes efficiently adsorbed FAM fluorophore-modified single-stranded DNA (ssDNA-FAM), quenching its fluorescence. In the presence of AFB1, the activator was released due to the preferential binding of the aptamer to AFB1, and the released activator then activated the trans-cleavage activity of Cas12a to indiscriminately cleave ssDNA on MXenes, leading to the recovery of the fluorescence signal. The fluorescent biosensor had a wide detection range from 0.001 to 80 ng mL, a detection limit of 0.92 pg mL, and the ability to detect within 80 min. More importantly, the platform demonstrates excellent detection performance in real peanut samples.
黄曲霉毒素 B1（AFB1）污染食品威胁全球食品安全，快速定量检测 AFB1 仍然是一个挑战。本文基于 CRISPR/Cas12a 和 MXenes 开发了一种用于 AFB1 检测的新型荧光生物传感器。具体来说，设计好的激活剂被双 AFB1 适体锁定，Cas12a 直接与 crRNA 连接形成失活复合物，而 MXenes 则能有效吸附 FAM 荧光标记的单链 DNA（ssDNA-FAM），使其荧光猝灭。在 AFB1 存在的情况下，由于适体优先与 AFB1 结合，激活剂被释放，释放的激活剂随后激活 Cas12a 的转切割活性，无差别地切割 MXenes 上的 ssDNA，从而恢复荧光信号。该荧光生物传感器的检测范围很宽，从 0.001 到 80 ng mL，检测限为 0.92 pg mL，检测时间在 80 min 以内。更重要的是，该平台在实际花生样品中表现出了优异的检测性能。
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