与小麦相关的新型类似病毒的分子特征
Molecular characterization of a novel virga-like virus associated with wheat.
机构信息
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.
National Agro-Technical Extension and Service Center, Beijing, 100125, China.
出版信息
Arch Virol. 2022 Sep;167(9):1909-1913. doi: 10.1007/s00705-022-05473-z. Epub 2022 Jun 25.
In this work, we report the detection of a novel single-strand RNA virus from wheat, tentatively named "Triticum aestivum-associated virga-like virus 1" (TaAVLV1). Further characterization revealed that the complete genome of TaAVLV1 is divided into two segments, RNA1 and RNA2, which are 3530 and 3466 nt in length, excluding their respective polyA tails, and each contains only one open reading frame (ORF). The ORF of RNA1 encodes an RNA-dependent RNA polymerase (RdRp), while the ORF of RNA2 encodes a putative protein with methyltransferase and helicase domains. Phylogenetic analysis showed that the RdRp of TaAVLV1 is closely related to those of members of the unclassified virga-like virus group in the family Virgaviridae. Thus, we have identified TaAVLV1 as a putative novel virga-like virus belonging to the family Virgaviridae.
在这项工作中，我们从小麦中检测到一种新型的单链 RNA 病毒，暂定名为“小麦伴枯斑病毒 1”（TaAVLV1）。进一步的特征分析表明，TaAVLV1 的完整基因组分为两个片段，RNA1 和 RNA2，分别长 3530 和 3466 个核苷酸，不包括它们各自的 polyA 尾巴，每个片段只包含一个开放阅读框（ORF）。RNA1 的 ORF 编码 RNA 依赖的 RNA 聚合酶（RdRp），而 RNA2 的 ORF 编码一个具有甲基转移酶和解旋酶结构域的假定蛋白。系统发育分析表明，TaAVLV1 的 RdRp 与丝状病毒科未分类的枯斑病毒组的成员密切相关。因此，我们将 TaAVLV1 鉴定为一种假定的新型枯斑样病毒，属于丝状病毒科。
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