State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.
Virology. 2021 Apr;556:101-109. doi: 10.1016/j.virol.2021.01.018. Epub 2021 Feb 5.
Wheat yellow mosaic virus (family Potyviridae; genus Bymovirus), is an important soil-borne virus that causes serious economic losses in wheat. In this study, we constructed infectious cDNA clones of WYMV genomic RNAs under the control of 35S or SP6 promoter for versatile usage (agroinfiltration or in vitro RNA transcription). Our results showed that an Agrobacterium-mediated inoculation system enabled WYMV to infect the leaves of Nicotiana benthamiana without causing WYMV systemic infection. However, in vitro transcripts from infectious cDNA clones using the SP6 promoter promoted WYMV systemic infection of wheat plants, which was then developed for further assays. The optimal temperature for virus multiplication and systemic infection of wheat was 8 °C. Additionally, a synergistic effect between WYMV and Chinese wheat mosaic virus (CWMV) was also detected. This is the first report of the construction of a Chinese isolate of WYMV and should facilitate the investigation of viral pathogenesis.
小麦黄花叶病毒（家族 Potyviridae；属 Bymovirus）是一种重要的土传病毒，可导致小麦严重的经济损失。在这项研究中，我们构建了在 35S 或 SP6 启动子控制下的 WYMV 基因组 RNA 的传染性 cDNA 克隆，以便于灵活使用（根癌农杆菌介导接种或体外 RNA 转录）。我们的结果表明，根癌农杆菌介导的接种系统可使 WYMV 感染不引起 WYMV 系统感染的本氏烟叶片。然而，使用 SP6 启动子的传染性 cDNA 克隆的体外转录促进了 WYMV 对小麦植株的系统感染，随后进行了进一步的检测。病毒增殖和小麦系统感染的最佳温度为 8°C。此外，还检测到 WYMV 和中国小麦花叶病毒（CWMV）之间的协同作用。这是首例中国 WYMV 分离株的构建报告，应有助于病毒发病机制的研究。
