Department of Ecological and Biological Sciences, Tuscia University, Viale dell'Università s/n, 01100, Viterbo, Italy.
Department of Experimental Medicine, "Sapienza" University of Rome, P.le Aldo Moro, 5, 00185, Rome, Italy.
BMC Res Notes. 2022 Jun 25;15(1):223. doi: 10.1186/s13104-022-06099-9.
Anisakis pegreffii is a zoonotic parasite requiring marine organisms to complete its life-history. Human infection (anisakiasis) occurs when the third stage larvae (L3) are accidentally ingested with raw or undercooked infected fish or squids. A new de novo transcriptome of A. pegreffii was here generated aiming to provide a robust bulk of data to be used for a comprehensive "ready-to-use" resource for detecting functional studies on genes and gene products of A. pegreffii involved in the molecular mechanisms of parasite-host interaction.
A RNA-seq library of A. pegreffii L3 was here newly generated by using Illumina TruSeq platform. It was combined with other five RNA-seq datasets previously gathered from L3 of the same species stored in SRA of NCBI. The final dataset was analyzed by launching three assembler programs and two validation tools. The use of a robust pipeline produced a high-confidence protein-coding transcriptome of A. pegreffii. These data represent a more robust and complete transcriptome of this species with respect to the actually existing resources. This is of importance for understanding the involved adaptive and immunomodulatory genes implicated in the "cross talk" between the parasite and its hosts, including the accidental one (humans).
海兽胃线虫是一种需要海洋生物才能完成其生命周期的人畜共患寄生虫。当第三期幼虫（L3）被意外摄入生的或未煮熟的受感染的鱼或鱿鱼时，就会发生人类感染（异尖线虫病）。本研究旨在生成海兽胃线虫的新从头转录组，为检测涉及寄生虫-宿主相互作用分子机制的海兽胃线虫基因和基因产物的功能研究提供大量可靠数据，以建立一个全面的“即用型”资源。
本研究使用 Illumina TruSeq 平台生成了海兽胃线虫 L3 的新 RNA-seq 文库，并与之前从同一物种的 L3 中收集并存储在 NCBI 的 SRA 中的另外五个 RNA-seq 数据集进行了组合。最终数据集通过启动三个组装程序和两个验证工具进行了分析。使用稳健的管道生成了海兽胃线虫的高可信度蛋白编码转录组。与现有的资源相比，这些数据代表了该物种更稳健和完整的转录组。这对于理解涉及寄生虫与其宿主之间“串扰”的适应性和免疫调节基因（包括意外宿主人类）非常重要。
