Department of Preventive Medicine, College of Medicine, Seoul National University,103 Daehak-ro, Jongno gu, Seoul 110-799, South Korea.
Department of Preventive Medicine, College of Medicine, Seoul National University,103 Daehak-ro, Jongno gu, Seoul 110-799, South Korea; Integrated Major in Innovative Medical Science, Seoul National University Graduate School, Seoul, South Korea.
Sci Total Environ. 2022 Oct 15;843:156938. doi: 10.1016/j.scitotenv.2022.156938. Epub 2022 Jun 23.
Epigenetic aging is associated with harmful health effects such as oxidative stress from heavy metal exposure. We considered the relationship between genes and heavy metals in association with oxidative stress and then investigated the association between serum α- klotho and lead and cadmium exposure among adults in the United States from 2007 to 2016 participating in the National Health and Nutrition Examination Survey (NHANES). Samples included 9800 adults aged 40 to 79 years with measurements of serum α-klotho, lead and cadmium, and complete covariate data. Lead and cadmium levels were measured by inductively coupled plasma mass spectrometry and serum α-klotho levels were measured using enzyme-linked immunosorbent assay (ELISA). Multivariate linear regression analysis was used to estimate the association between serum α-klotho and blood lead, blood cadmium, and urinary cadmium. A percent increase in blood lead, blood cadmium, and urinary cadmium was associated with a statistically significant 4.0 % (p < 0.001), 2.0 %, (p = 0.003) and 1.0 % (p = 0.020) decrease in serum klotho. After adjustment, a percent increase in blood lead was associated with a statistically significant 4.0 % (p < 0.001) decrease in serum klotho; blood and urinary cadmium did not show any statistically significant associations after adjustment (β (95 % CI), p-value for blood cadmium: 0.00 (-0.02-0.01), p = 0.573; urinary cadmium: -0.01 (-0.03-0.01), p = 0.210). Mean serum klotho levels showed a statistically significant decreasing trend with increasing blood lead quartiles (unadjusted and all-adjusted geometric means and 95 % confidence intervals of serum klotho (in pg/mL) for Q1, Q2, Q3, and Q4: unadjusted: 827.49 (814.20-840.92), 811.92 (794.73-829.48), 791.48 (775.11-808.19), and 772.01 (754.23-790.29); adjusted: 830.64 (805.53-856.45), 816.07 (789.18-843.87), 800.71 (773.71-828.57), and 784.31 (757.94-811.59)). Blood lead and levels were negatively associated with serum α-klotho levels in a representative population of US adults. These results suggest that blood lead levels may be associated with the serum levels of a protein associated with cognition and aging. Further research is recommended to investigate the causality behind such relationship.
表观遗传衰老与有害的健康影响有关，例如重金属暴露引起的氧化应激。我们考虑了基因与重金属与氧化应激之间的关系，然后研究了 2007 年至 2016 年期间参加美国国家健康和营养检查调查（NHANES）的成年人血清α-klotho 与铅和镉暴露之间的关系。样本包括 9800 名 40 至 79 岁的成年人，他们的血清α-klotho、铅和镉以及完整的协变量数据都有测量。通过电感耦合等离子体质谱法测量铅和镉水平，使用酶联免疫吸附测定法（ELISA）测量血清α-klotho 水平。使用多元线性回归分析来估计血清α-klotho 与血铅、血镉和尿镉之间的关联。血铅、血镉和尿镉的百分比增加与血清 klotho 统计学上显著的 4.0%（p < 0.001）、2.0%（p = 0.003）和 1.0%（p = 0.020）降低相关。调整后，血铅的百分比增加与血清 klotho 统计学上显著的 4.0%（p < 0.001）降低相关；血镉和尿镉在调整后没有显示出任何统计学上显著的关联（β（95%CI），p 值血镉：0.00（-0.02-0.01），p = 0.573；尿镉：-0.01（-0.03-0.01），p = 0.210）。血清 klotho 水平的平均值显示出随着血铅四分位的增加而呈统计学显著下降趋势（未经调整和所有调整的几何平均值和血清 klotho 的 95%置信区间（pg/mL）为 Q1、Q2、Q3 和 Q4：未经调整：827.49（814.20-840.92），811.92（794.73-829.48），791.48（775.11-808.19）和 772.01（754.23-790.29）；调整后：830.64（805.53-856.45），816.07（789.18-843.87），800.71（773.71-828.57）和 784.31（757.94-811.59））。血铅和水平与美国成年人代表性人群中的血清α-klotho 水平呈负相关。这些结果表明，血铅水平可能与认知和衰老相关的蛋白的血清水平有关。建议进行进一步的研究，以调查这种关系背后的因果关系。
