Gan Lin, Wang Jiaoyang, Qu Kang, Jiang Wei, Lei Yuhong, Dong Ming
Department of Neurology and Neuroscience Center, The First Hospital of Jilin University, Changchun, China.
Cancer Institute, The First Hospital of Jilin University, Changchun, China.
Front Public Health. 2025 Mar 19;13:1457630. doi: 10.3389/fpubh.2025.1457630. eCollection 2025.
Acrylamide (AA) is a ubiquitous environmental contaminant linked to systemic inflammation and oxidative stress in animal studies; however, the epidemiological evidence is still lacking. This study aimed to evaluate the association of AA exposure with markers of systemic inflammation and serum concentrations of an anti-aging protein, -klotho.
The study used data of 1,545 adults aged 40-79 years from the National Health and Nutrition Examination Survey (NHANES) 2013-2016. Internal AA exposure was assessed using hemoglobin adducts of acrylamide and glycidamide (HbAA and HbGA, respectively), the sum of HbAA and HbGA (HbAA + HbGA), and the ratio of HbGA and HbAA (HbGA/HbAA). Two novel indicators, systemic immune-inflammation index (SII) and system inflammation response index (SIRI), were calculated using the lymphocyte, platelet, neutrophil, and monocyte counts. The serum concentration of soluble -klotho was measured using enzyme-linked immunosorbent assay. Multivariable linear regression models were used to estimate the associations of AA hemoglobin biomarkers with systemic inflammation indicators and serum concentration of -klotho.
Each one-unit increase in ln-transformed HbAA, HbGA, and HbAA+HbGA was associated with an increase in SII in models adjusted for age, sex, and race/ethnicity [regression coefficient () = 32.16, 95% confidence interval (CI): 3.59, 60.73; β =36.37, 95% CI: 5.59, 67.15; and β = 37.17, 95% CI: 6.79, 67.55, respectively]. However, the associations were no longer significant after additional adjustment for lifestyle factors. Higher HbAA and HbAA+HbGA predicted lower serum -klotho concentrations ( = -35.76 pg./mL, 95% CI: -63.27, -8.25; β = -33.82 pg./mL, 95% CI: -62.68, -4.96, respectively).
The hemoglobin adducts of AA parameters, as biomarkers of internal AA exposure, were associated with reduced serum concentrations of -klotho among the United States population in their middle-late adulthood. The findings indicated that exposure to AA may have impacts on the molecular pathways of aging and related diseases by influencing -klotho concentrations.
丙烯酰胺（AA）是一种普遍存在的环境污染物，在动物研究中与全身炎症和氧化应激有关；然而，流行病学证据仍然缺乏。本研究旨在评估AA暴露与全身炎症标志物以及一种抗衰老蛋白——β-klotho血清浓度之间的关联。
该研究使用了2013 - 2016年美国国家健康与营养检查调查（NHANES）中1545名40 - 79岁成年人的数据。通过丙烯酰胺和环氧丙酰胺的血红蛋白加合物（分别为HbAA和HbGA）、HbAA与HbGA之和（HbAA + HbGA）以及HbGA与HbAA之比（HbGA/HbAA）来评估体内AA暴露情况。使用淋巴细胞、血小板、中性粒细胞和单核细胞计数计算两个新的指标，即全身免疫炎症指数（SII）和全身炎症反应指数（SIRI）。采用酶联免疫吸附测定法测量可溶性β-klotho的血清浓度。使用多变量线性回归模型来估计AA血红蛋白生物标志物与全身炎症指标以及β-klotho血清浓度之间的关联。
在根据年龄、性别和种族/民族进行调整的模型中，ln转换后的HbAA、HbGA和HbAA + HbGA每增加一个单位，SII均升高[回归系数（β）分别为= 32.16，95%置信区间（CI）：3.59，60.73；β = 36.37，95% CI：5.59，67.15；β = 37.17，95% CI：6.79，67.55]。然而，在进一步调整生活方式因素后，这些关联不再显著。较高的HbAA和HbAA + HbGA预示着较低的血清β-klotho浓度（分别为β = -35.76 pg./mL，95% CI：-63.27，-8.25；β = -33.82 pg./mL，95% CI：-62.68，-4.96）。
在美国中老年人群中，作为体内AA暴露生物标志物的AA参数血红蛋白加合物与较低的血清β-klotho浓度相关。研究结果表明，AA暴露可能通过影响β-klotho浓度对衰老和相关疾病的分子途径产生影响。
