De Marzo Davide, Losacco Caterina, Laudadio Vito, Tufarelli Vincenzo, Xiong Youling L
Department of Precision and Regenerative Medicine and Jonian Area, Section of Veterinary Science and Animal Production, University of Bari 'Aldo Moro', 70010 Valenzano, Italy.
Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA.
Foods. 2023 Mar 17;12(6):1284. doi: 10.3390/foods12061284.
The muscle and adipose tissue histological properties in wether and ewe lambs of Gentile di Puglia breed, fed diets including two protein sources [soybean meal (SB) and SB plus distillers dried grain with solubles (DD)] and three protein levels (12.5, 15.7, and 18.9%) were evaluated. Muscle samples were collected from the longissimus/rump, cut, and stained (reciprocal aerobic and anaerobic stains) for muscle fiber typing and fat cell characterization. Fibers were classified as α-red, β-red, and α-white. Lambs fed SB had larger α-white ( < 0.10) and smaller-diameter β-red and α-red fibers ( < 0.05). Among dietary protein levels, lambs fed 12.5% protein exhibited the highest percentage of α-red and the greatest diameter of α-white fibers, whereas wethers had a higher percentage of α-red ( < 0.05), and ewes had a higher percentage of α-white fibers ( < 0.05). Intramuscular fat cells were larger ( < 0.10) in ewes than in wethers. Lambs in the group fed 12.5% protein had larger subcutaneous fat cells at the sacral vertebrae location. Overall, both sources and levels of dietary protein had significant effects on lamb muscle and fat histological features, suggesting the potential of modulating muscle or fiber types through dietary protein strategies.
对饲养在普利亚大区詹蒂莱品种的阉公羊和母羊羔，饲喂包含两种蛋白质来源（豆粕（SB）以及SB加干酒糟及其可溶物（DD））和三种蛋白质水平（12.5%、15.7%和18.9%）日粮的肌肉和脂肪组织的组织学特性进行了评估。从背最长肌/臀部采集肌肉样本，进行切割，并进行染色（互作有氧和无氧染色），以进行肌纤维分型和脂肪细胞表征。纤维被分类为α-红色、β-红色和α-白色。饲喂SB的羔羊具有更大的α-白色纤维（<0.10）以及直径更小的β-红色和α-红色纤维（<0.05）。在日粮蛋白质水平中，饲喂12.5%蛋白质的羔羊表现出最高比例的α-红色纤维和最大直径的α-白色纤维，而阉公羊具有更高比例的α-红色纤维（<0.05）且母羊具有更高比例的α-白色纤维（<0.05）。母羊的肌内脂肪细胞比阉公羊更大（<0.10）。饲喂12.5%蛋白质组的羔羊在骶椎位置具有更大的皮下脂肪细胞。总体而言，日粮蛋白质的来源和水平均对羔羊肌肉和脂肪的组织学特征有显著影响，表明通过日粮蛋白质策略调节肌肉或纤维类型具有潜力。
