Nyby J G, Simon N G
Physiol Behav. 1987;39(2):147-51. doi: 10.1016/0031-9384(87)90003-5.
Castrated DBA/2J male mice emitted 70 kHz vocalizations to female stimuli in response to 10 days of treatment with either testosterone (T, 300 micrograms/day), diethylstilbestrol (DES, 1 or 3 micrograms/day) or methyltrienolone (R1881, 900 micrograms/day). Lower dosages of R1881 (300 and 600 micrograms/day) and the oil vehicle were relatively ineffective in restoring vocalizations. The effects of these hormones on restoring seminal vesicle weight did not always parallel their effects upon behavior. In general T and R1881 (600 and 900 micrograms/day) were effective in restoring seminal vesicles while DES, the lowest dose of R1881 (300 micrograms/day), and the oil vehicle were ineffective. In receptor competition studies, R1881 pretreatment significantly reduced estrogen binding in hypothalamic-preoptic cytosol. In fact the most effective dose for restoring vocalizations (900 micrograms/day) reduced available estrogen binding sites by 91%. We propose that the male-typical vocalizations of mice may normally be stimulated through the activation of estrogen receptors following androgen aromatization and that the ability of a pharmacological dosage of R1881 (900 micrograms/day) to restore behavior may be due to interaction with estrogen receptors in the brain.
去势的DBA/2J雄性小鼠在接受睾酮（T，300微克/天）、己烯雌酚（DES，1或3微克/天）或甲基三烯醇酮（R1881，900微克/天）治疗10天后，会对雌性刺激发出70千赫的叫声。较低剂量的R1881（300和600微克/天）以及油剂载体在恢复叫声方面相对无效。这些激素对恢复精囊重量的影响并不总是与其对行为的影响平行。一般来说，T和R1881（600和900微克/天）能有效恢复精囊，而DES、最低剂量的R1881（300微克/天）和油剂载体则无效。在受体竞争研究中，R1881预处理显著降低了下丘脑 - 视前区胞质溶胶中的雌激素结合。事实上，恢复叫声最有效的剂量（900微克/天）使可用的雌激素结合位点减少了91%。我们提出，小鼠的雄性典型叫声可能通常是通过雄激素芳香化后激活雌激素受体来刺激的，并且药理剂量的R1881（900微克/天）恢复行为的能力可能是由于与大脑中的雌激素受体相互作用。
