Zhan Yawei, Wang Meixin, Ma Tengfei, Li Zhiqiang
International Centre for Bamboo and Rattan, Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and Technology Beijing 100102 China
RSC Adv. 2022 Jun 7;12(26):16942-16954. doi: 10.1039/d2ra02421g. eCollection 2022 Jun 1.
In this study, the effect of the γ-valerolactone (GVL)/HO pretreatment system on bamboo () for enzymatic hydrolysis and ethanol fermentation was investigated. The performance characterization of the pretreated bamboo substrates, including the chemical composition, the structural characteristics, and the ability to produce bioethanol, were evaluated. The recovered substrates were enzymatically hydrolyzed for 48 h and then fermented to bioethanol. For the cellulose in the raw bamboo material, the highest cellulose-to-glucose conversion yield (CGCY) was achieved at 140 °C for 2 h with GVL : HO = 8 : 2, which was 73.39%, and the cellulose-to-ethanol conversion yield (CECY) was 67.00%. This indicated that 183.5 kg of bioethanol could be produced per ton of bamboo, which was 9.71-folds higher than that directly converted from the untreated raw bamboo powder. Under these conditions, 50.60% of the active lignin can be recovered and be used as a wood-derived feedstock for further high-valued utilization. Meanwhile, the maximum concentration of fermentation inhibitors formed after pretreatment was about 140.9 mmol L, and had weak inhibition to the subsequent reaction. It has been shown that the cellulose could be effectively separated from bamboo and converted into bioethanol through the GVL/HO pretreatment system.
在本研究中，研究了γ-戊内酯（GVL）/水预处理体系对竹子（）进行酶水解和乙醇发酵的效果。对预处理后的竹子底物进行了性能表征，包括化学成分、结构特征以及生产生物乙醇的能力。将回收的底物进行48小时的酶水解，然后发酵成生物乙醇。对于生竹原料中的纤维素，在140℃下用GVL∶水 = 8∶2处理2小时时，纤维素到葡萄糖的转化率（CGCY）最高，为73.39%，纤维素到乙醇的转化率（CECY）为67.00%。这表明每吨竹子可生产183.5千克生物乙醇，比未经处理的生竹粉直接转化的产量高9.71倍。在这些条件下，50.60%的活性木质素可以被回收，并用作木材衍生原料进行进一步的高价值利用。同时，预处理后形成的发酵抑制剂的最大浓度约为140.9 mmol/L，对后续反应的抑制作用较弱。结果表明，通过GVL/水预处理体系可以有效地从竹子中分离出纤维素并将其转化为生物乙醇。
