Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, College of Future Technology, Peking University, Beijing, China.
Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, College of Future Technology, Peking University, Beijing, China; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
STAR Protoc. 2022 Jun 17;3(3):101480. doi: 10.1016/j.xpro.2022.101480. eCollection 2022 Sep 16.
The communication between macrophage and adipocyte plays a critical role in the initiation and development of metabolic inflammation, which is difficult to study . Here, we provide a step-by-step protocol using differentiated cells to investigate the paracrine effects of classically activated macrophage on beige adipocyte metabolism . This protocol uses bone-marrow-derived macrophage and SVF-derived UCP1 beige adipocyte in a culture model to study immune regulation of adipocyte metabolism by western blot analyses. For complete details on the use and execution of this protocol, please refer to Yao et al. (2021).
巨噬细胞和脂肪细胞之间的通讯在代谢性炎症的发生和发展中起着关键作用，但这种通讯机制很难研究。在这里，我们提供了一个使用分化细胞的分步方案，用于研究经典激活的巨噬细胞对米色脂肪细胞代谢的旁分泌作用。该方案使用骨髓来源的巨噬细胞和 SVF 来源的 UCP1 米色脂肪细胞在培养模型中，通过 Western blot 分析研究脂肪细胞代谢的免疫调节。有关该方案使用和执行的完整详细信息，请参考 Yao 等人。（2021）。
