Ren Yanling, Wang Tao, Jiang Yingjie, Chen Pengchao, Tang Jian, Wang Juan, Jin Daochao, Guo Jianjun
Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Scientific Observing and Experimental Station of Crop Pest in Guiyang, Ministry of Agriculture, Guizhou University, Guiyang, China.
Guizhou Light Industry Technical College, Guiyang, China.
Front Chem. 2022 Jun 8;10:921113. doi: 10.3389/fchem.2022.921113. eCollection 2022.
In this study, four kinds of chemical substances (2,3,5,6-tetramethylpyrazine, -ionone, citronellal, and paeonol), three kinds of plant essential oils (tea tree essential oil, lavender essential oil, and myrrh essential oil), and their combinations were selected to explore their synergistic effects on tobacco beetle [ (Fabricius) (Coleoptera: Anobiidae)] adults by the behavioral test and laboratory simulation test. Behavioral test results showed that some of the combinations revealed a synergistic effect on tobacco beetle adults, especially the sexual attractant +2,3,5,6-tetramethylpyrazine + -ionone + citronellal + paeonol (SABCD, one portion of sexual attractant, and 1 mg/L synergistic substances) combination and the food attractant +2,3,5,6-tetramethylpyrazine + paeonol (FAD, 1 ml of food attractant and 1 mg/L synergistic substances) combination showed the best behavioral effect on tobacco beetle adults with average dwell times of 120.97 and 126.74 s, respectively, compared to those of other combinations. Meanwhile, SABCD had the highest selection rate [89.47%, about 1.5 times that of the sexual attractant (S)] on tobacco beetle adults compared with those of other combinations. In addition, laboratory simulation test results showed that the SABCD combination had the highest average selection rate (37.31%, about 2 times that of S) on tobacco beetle adults at 1 mg/L. However, our results showed that there was no significant difference in the indoor simulation results of food attractant synergistic substances. Our results will provide guidance for the development of new pesticides for tobacco beetle adults.
在本研究中，选取了四种化学物质（2,3,5,6 - 四甲基吡嗪、β - 紫罗兰酮、香茅醛和丹皮酚）、三种植物精油（茶树精油、薰衣草精油和没药精油）及其组合，通过行为测试和实验室模拟测试来探究它们对烟草甲[Lasioderma serricorne (Fabricius)（鞘翅目：窃蠹科）]成虫的协同作用。行为测试结果表明，部分组合对烟草甲成虫表现出协同作用，尤其是性引诱剂 + 2,3,5,6 - 四甲基吡嗪 + β - 紫罗兰酮 + 香茅醛 + 丹皮酚（SABCD，一份性引诱剂和1 mg/L协同物质）组合以及食物引诱剂 + 2,3,5,6 - 四甲基吡嗪 + 丹皮酚（FAD，1 ml食物引诱剂和1 mg/L协同物质）组合对烟草甲成虫表现出最佳行为效果，平均停留时间分别为120.97和126.74 s，优于其他组合。同时，与其他组合相比，SABCD对烟草甲成虫的选择率最高[89.47%，约为性引诱剂（S）的1.5倍]。此外，实验室模拟测试结果表明，SABCD组合在1 mg/L时对烟草甲成虫的平均选择率最高（37.31%，约为S的2倍）。然而，我们的结果表明食物引诱剂协同物质的室内模拟结果无显著差异。我们的研究结果将为开发针对烟草甲成虫的新型农药提供指导。
