Han Jintao, Lu Chunyang, Shen Mengzhu, Sun Xiaoyi, Mo Xiaodong, Yang Gen
State Key Laboratory of Nuclear Physics and Technology, School of Physics, Peking University, Beijing 100871, China.
Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China.
ACS Omega. 2022 Jun 8;7(24):20761-20767. doi: 10.1021/acsomega.2c01153. eCollection 2022 Jun 21.
Isolation of circulating tumor cells (CTCs) is of great significance for the diagnosis, prognosis, and treatment of metastatic cancer. Among CTC capture methods independent of antibodies, membrane filtration-based methods have the advantages of simplicity, rapidity, and high throughput but usually have problems such as clogging, high pressure drop, and impaired cell viability. In this study, we designed and tested a reusable device that used horizontal rotor and fluid-assisted separation to capture CTCs by centrifugal membrane filtration, achieving simple, fast, highly efficient, and viable cell capture on traditional centrifuge. The average capture efficiency was 95.8% for different types of cancer cells with >90% survival, and the removal of white blood cells can reach 99.72% under four times cleaning of the membrane after filtration. A further clinic demo was performed using the device to detect residual leukemic cells in patients; the results showed a 10-fold enrichment of the leukemic cells in peripheral blood samples. Taken together, the simple, robust, and efficient CTC capture device may have the potential for clinic routine detection and analysis of circulating tumor cells.
循环肿瘤细胞（CTC）的分离对于转移性癌症的诊断、预后评估和治疗具有重要意义。在不依赖抗体的CTC捕获方法中，基于膜过滤的方法具有操作简单、速度快和高通量的优点，但通常存在堵塞、高压降和细胞活力受损等问题。在本研究中，我们设计并测试了一种可重复使用的装置，该装置利用水平转子和流体辅助分离，通过离心膜过滤捕获CTC，在传统离心机上实现了简单、快速、高效且能保持细胞活力的捕获。对于不同类型的癌细胞，平均捕获效率为95.8%，细胞存活率>90%，过滤后对膜进行四次清洗，白细胞去除率可达99.72%。使用该装置对患者进行了进一步的临床演示，以检测残留的白血病细胞；结果显示外周血样本中的白血病细胞富集了10倍。综上所述，这种简单、耐用且高效的CTC捕获装置可能具有用于临床常规检测和分析循环肿瘤细胞的潜力。
