Tingan Thomas Koge, Mensah Gloria Ivy, Agyekum Edward Bensa, Amanor Ivy Brago, Addo Samuel Ofori, Ayamdoo Yolanda Isabel, Duah Mabel Sarpong, Mosi Lydia, Addo Kennedy Kwasi
West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences, University of Ghana, Legon, Accra, Ghana.
School of Veterinary Medicine, College of Basic and Applied Sciences, University of Ghana, Legon, Accra, Ghana.
IJID Reg. 2022 Feb 11;3:8-14. doi: 10.1016/j.ijregi.2022.02.004. eCollection 2022 Jun.
The aim was to isolate and identify the species of mycobacteria causing tuberculous-like (TB-like) lesions in cattle in Ghana.
Between 2019 and 2020, 68 bovine tissue samples with TB-like lesions, identified during post slaughter examination, were obtained from four major abattoirs close to border towns in Ghana. The samples were cultured on Lowenstein-Jensen medium. Isolated bacteria were characterized by Ziehl-Neelsen staining and observation for acid-fast bacilli (AFB) under a microscope. DNA was extracted from AFB-positive isolates, and mycobacterial speciation was performed by line probe assay using GenoType CM and also with mycobacterial 16S rRNA gene amplification and sequencing.
No was identified; however 53 bacterial isolates were obtained, of which 41 were non-tuberculous mycobacteria (NTM) strains and 12 were gram-positive bacteria. The predominant NTM species was (43.9%, 18/41), with the rest being , and .
In cattle, NTM contribute significantly to lesions observed during slaughter examination and may be an important cause of zoonotic tuberculosis. A One Health surveillance of NTM in Ghana would provide insights into their clinical significance.
旨在分离并鉴定在加纳牛群中引起类结核（TB样）病变的分枝杆菌种类。
在2019年至2020年期间，从加纳边境城镇附近的四个主要屠宰场获取了68份在屠宰后检查中鉴定出的具有TB样病变的牛组织样本。样本在罗-琴培养基上培养。分离出的细菌通过萋-尼染色进行鉴定，并在显微镜下观察抗酸杆菌（AFB）。从AFB阳性分离株中提取DNA，并使用GenoType CM通过线性探针分析以及通过分枝杆菌16S rRNA基因扩增和测序进行分枝杆菌种类鉴定。
未鉴定出[此处原文缺失相关内容]；然而获得了53株细菌分离株，其中41株为非结核分枝杆菌（NTM）菌株，12株为革兰氏阳性菌。主要的NTM种类是[此处原文缺失相关内容]（43.9%，18/41），其余为[此处原文缺失相关内容]和[此处原文缺失相关内容]。
在牛群中，NTM对屠宰检查期间观察到的病变有显著贡献，可能是人畜共患结核病的一个重要原因。在加纳对NTM进行“同一健康”监测将有助于深入了解其临床意义。
