Singh Pratiksha, Tewari Ashish, Pandey Vivek
Plant Ecology and Climate Change Science Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh 226001 India.
Department of Forestry & Environmental Science, D.S.B. Campus, Kumaun University, Nainital, Uttarakhand 263001 India.
3 Biotech. 2022 Jul;12(7):152. doi: 10.1007/s13205-022-03215-1. Epub 2022 Jun 21.
Increasing concentrations of ground-level ozone (O) exert significant impacts on the plants, but there is limited data for belowground processes. We studied the effects of long-term exposure of elevated O (EO) on plant growth parameters (plant height and biomass) and biochemical parameters (nutrients, microbial biomass and enzymatic activities) of rhizospheric soil of leguminous tree species . seedlings were grown under ambient O (AO) and EO (+20 ppb above ambient) under Free Air Ozone Concentration Enrichment (O-FACE) facility and changes in plant growth and their rhizospheric soil properties were studied during 6, 12, 18 and 24 months of EO exposure showed significant reductions in shoot length, root biomass, shoot biomass, leaf biomass and total biomass during 12, 18 and 24 months of exposure to EO. Total nutrients in rhizospheric soil like carbon and phosphorus were significantly reduced after 24 months of EO exposure. Most of the available nutrients showed significant reduction after 6, 12 and 24 months of EO exposure. A significant decrease was apparent in microbial biomass carbon, nitrogen and phosphorus after 6, 12, 18 and 24 months of EO treatment. Significant reductions were observed in extracellular enzymatic activities (dehydrogenase, alkaline phosphatase, -glycosidase, fluorescein diacetate, arylsulfatase, cellulase and protease) of soil after 6, 12 and 24 months of EO exposure. These results suggest that increasing O concentrations will directly impact growth as well as have indirect impact on the nutrient contents (C, N, and P), microbial biomass and extracellular enzymatic activities of rhizospheric soil of . Our results suggest that continuous increase in O concentrations will have serious implications for aboveground plant growth and belowground soil fertility in this region considered as O hotspot.
The online version contains supplementary material available at 10.1007/s13205-022-03215-1.
地面臭氧（O）浓度增加对植物有显著影响，但关于地下过程的数据有限。我们研究了长期暴露于升高的臭氧（EO）对豆科树种根际土壤的植物生长参数（株高和生物量）和生化参数（养分、微生物生物量和酶活性）的影响。幼苗在自由空气臭氧浓度富集（O-FACE）设施下于环境臭氧（AO）和EO（比环境浓度高20 ppb）条件下生长，并在EO暴露6、12、18和24个月期间研究植物生长及其根际土壤性质的变化。EO暴露12、18和24个月期间，茎长、根生物量、茎生物量、叶生物量和总生物量显著降低。EO暴露24个月后，根际土壤中的总养分如碳和磷显著减少。大多数有效养分在EO暴露6、12和24个月后显著减少。EO处理6、12、18和24个月后，微生物生物量碳、氮和磷明显减少。EO暴露6、12和24个月后，土壤的细胞外酶活性（脱氢酶、碱性磷酸酶、β-糖苷酶、荧光素二乙酸酯、芳基硫酸酯酶、纤维素酶和蛋白酶）显著降低。这些结果表明，臭氧浓度增加将直接影响[豆科树种名称]的生长，并对其根际土壤的养分含量（碳、氮和磷）、微生物生物量和细胞外酶活性产生间接影响。我们的结果表明，在这个被视为臭氧热点的地区，臭氧浓度的持续增加将对地上植物生长和地下土壤肥力产生严重影响。
在线版本包含可在10.1007/s13205-022-03215-1获取的补充材料。
