Yasar Yildiz Songul, Finore Ilaria, Leone Luigi, Romano Ida, Lama Licia, Kasavi Ceyda, Nicolaus Barbara, Toksoy Oner Ebru, Poli Annarita
Department of Bioengineering, Istanbul Medeniyet University, Istanbul, Turkey.
Institute of Biomolecular Chemistry (ICB), National Research Council, Naples, Italy.
Front Microbiol. 2022 Jun 9;13:923038. doi: 10.3389/fmicb.2022.923038. eCollection 2022.
strain M1 is a Gram-positive, motile, facultative anaerobic, spore forming, and thermophilic bacterium, isolated from geothermal soil of the crater of Mount Melbourne (74°22' S, 164°40' E) during the Italian Antarctic Expedition occurred in Austral summer 1986-1987. Strain M1 demonstrated great biotechnological and industrial potential owing to its ability to produce exopolysaccharides (EPSs), ethanol and thermostable extracellular enzymes, such as an xylanase and a β-xylosidase, and intracellular ones, such as xylose/glucose isomerase and protease. Furthermore, recent studies revealed its high potential in green chemistry due to its use in residual biomass transformation/valorization and as an appropriate model for microbial astrobiology studies. In the present study, using a systems-based approach, genomic analysis of M1 was carried out to enlighten its functional characteristics. The elucidation of whole-genome organization of this thermophilic cell factory increased our understanding of biological mechanisms and pathways, by providing valuable information on the essential genes related to the biosynthesis of nucleotide sugar precursors, monosaccharide unit assembly, as well as the production of EPSs and ethanol. In addition, gene prediction and genome annotation studies identified genes encoding xylanolytic enzymes that are required for the conversion of lignocellulosic materials to high-value added molecules. Our findings pointed out the significant potential of strain M1 in various biotechnological and industrial applications considering its capacity to produce EPSs, ethanol and thermostable enzymes the utilization of lignocellulosic waste materials.
菌株M1是一种革兰氏阳性、具运动性、兼性厌氧、产芽孢且嗜热的细菌，于1986 - 1987年南半球夏季意大利南极考察期间，从墨尔本山火山口（南纬74°22′，东经164°40′）的地热土壤中分离得到。菌株M1展现出巨大的生物技术和工业潜力，因为它能够产生胞外多糖（EPSs）、乙醇以及热稳定的胞外酶，如木聚糖酶和β - 木糖苷酶，还有胞内酶，如木糖/葡萄糖异构酶和蛋白酶。此外，最近的研究表明，由于其在残余生物质转化/增值方面的应用以及作为微生物天体生物学研究的合适模型，它在绿色化学领域具有很高的潜力。在本研究中，采用基于系统的方法对M1进行了基因组分析，以阐明其功能特性。对这个嗜热细胞工厂全基因组组织的阐明，通过提供与核苷酸糖前体生物合成、单糖单元组装以及EPSs和乙醇生产相关的必需基因的有价值信息，增加了我们对生物机制和途径的理解。此外，基因预测和基因组注释研究确定了编码木聚糖分解酶的基因，这些酶是将木质纤维素材料转化为高附加值分子所必需的。我们的研究结果指出，考虑到菌株M1产生EPSs、乙醇和热稳定酶的能力以及对木质纤维素废料的利用，它在各种生物技术和工业应用中具有巨大潜力。
