Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio av. 7, LT-10257 Vilnius, Lithuania.
Department of Microbiology and Biotechnology, Institute of Bioscience, Life Sciences Center, Vilnius University, Saulėtekio av. 7, LT-10257 Vilnius, Lithuania.
Int J Mol Sci. 2023 Sep 12;24(18):13980. doi: 10.3390/ijms241813980.
A high temperature-adapted bacteriophage, vB_PtoS_NIIg3.2 (NIIg3.2), was isolated in Lithuania from compost heaps using strain NIIg-3 as a host for phage propagation. Furthermore, NIIg3.2 was active against four strains of , and it infected the host cells from 50 to 80 °C. Transmission electron microscopy analysis revealed siphovirus morphology characterized by an isometric head (59 nm in diameter) and a noncontractile tail (226 nm in length). The double-stranded DNA genome of NIIg3.2 (38,970 bp) contained 71 probable protein-encoding genes and no genes for tRNA. In total, 29 NIIg3.2 ORFs were given a putative functional annotation, including those coding for the proteins responsible for DNA packaging, virion structure/morphogenesis, phage-host interactions, lysis/lysogeny, replication/regulation, and nucleotide metabolism. Based on comparative phylogenetic and bioinformatic analysis, NIIg3.2 cannot be assigned to any genus currently recognized by ICTV and potentially represents a new one within siphoviruses. The results of this study not only extend our knowledge about poorly explored thermophilic bacteriophages but also provide new insights for further investigation and understanding the evolution of -group bacteria-infecting viruses.
一种高温适应噬菌体 vB_PtoS_NIIg3.2（NIIg3.2）在立陶宛从堆肥堆中分离出来，使用菌株 NIIg-3 作为噬菌体繁殖的宿主。此外，NIIg3.2 对四种 菌株具有活性，它可以在 50 到 80°C 感染宿主细胞。透射电子显微镜分析显示出丝状噬菌体的形态特征，具有等径头部（59nm 直径）和非收缩尾部（226nm 长度）。NIIg3.2 的双链 DNA 基因组（38970bp）包含 71 个可能的蛋白质编码基因，没有 tRNA 基因。总共，29 个 NIIg3.2 ORF 被赋予了推测的功能注释，包括那些编码负责 DNA 包装、病毒粒子结构/形态发生、噬菌体-宿主相互作用、裂解/溶原、复制/调控和核苷酸代谢的蛋白质。基于比较系统发育和生物信息学分析，NIIg3.2 不能分配到 ICTV 目前认可的任何属，并且可能代表丝状噬菌体中的一个新属。这项研究的结果不仅扩展了我们对研究较少的嗜热噬菌体的认识，还为进一步研究和理解感染 - 组细菌的病毒的进化提供了新的见解。
