Department of Stomatology, Shihezi University School of Medicine & the First Affiliated Hospital to Shihezi University School of Medicine, Shihezi, 832002 Xinjiang, China.
Department of Pathology, Northern Jiangsu People's Hospital Affiliated to Yangzhou University/Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225000, China.
Dis Markers. 2022 Jun 17;2022:7300593. doi: 10.1155/2022/7300593. eCollection 2022.
PURPOSE: Oral squamous cell carcinoma (OSCC) is the sixth leading cause of cancer-related death worldwide and is characterized by metastasis and recurrence. We aimed to evaluate the expression of AKT1 and PLK1 in OSCC and identify their correlation with the clinical and histological features and prognosis of patients with OSCC. METHODS: Tissue samples were collected from 70 patients with OSCC and 50 patients with normal oral mucosa. The expression levels of AKT1 and PLK1 in OSCC tissues and normal oral mucosa were detected by immunohistochemistry. The chi-square test was used to identify correlations between the expression levels of AKT1 and PLK1 with patients' clinicopathologic characteristics. Survival analysis was assessed by the Kaplan-Meier method. Spearman's rank correlation test was used to determine the relationships between AKT1 and PLK1 expressions. The bioinformatics database GEPIA was used to verify the experimental results. RESULTS: The chi-square test and Fisher's exact test showed that the positive expression rate of AKT1 and PLK1 in OSCC tissue was significantly higher than that in the normal oral mucosa ( < 0.05). PLK1 expression levels were significantly correlated with tumor stage and size ( < 0.05). Kaplan-Meier analysis showed that the survival time of AKT1 and PLK1 with high expression was significantly shorter than that of patients with low expression ( < 0.05). Spearman's rank correlation test showed a strong correlation between AKT1 and PLK1 expression in OSCC tissue ( = 0.53; < 0.05). GEPIA bioinformatics database analysis results show that the expression and overall survival of AKT1 and PLK1 analysis and the correlation analysis of AKT1 and PLK1 were consistent with experimental results. CONCLUSION: AKT1 and PLK1 expressions are associated with the occurrence and progression of OSCC and may be used as diagnostic and prognostic indicators of OSCC. There may be a correlation between AKT1 and PLK1 in OSCC tissue.
目的：口腔鳞状细胞癌（OSCC）是全球第六大癌症相关死亡原因，其特征为转移和复发。我们旨在评估 AKT1 和 PLK1 在 OSCC 中的表达，并确定其与 OSCC 患者的临床和组织学特征及预后的相关性。
方法：收集 70 例 OSCC 患者和 50 例正常口腔黏膜患者的组织样本。采用免疫组织化学法检测 OSCC 组织和正常口腔黏膜中 AKT1 和 PLK1 的表达水平。采用卡方检验分析 AKT1 和 PLK1 表达水平与患者临床病理特征的相关性。采用 Kaplan-Meier 法评估生存分析。采用 Spearman 秩相关检验分析 AKT1 和 PLK1 表达之间的关系。采用生物信息学数据库 GEPIA 验证实验结果。
结果：卡方检验和 Fisher 确切检验显示，OSCC 组织中 AKT1 和 PLK1 的阳性表达率明显高于正常口腔黏膜（ < 0.05）。PLK1 表达水平与肿瘤分期和大小显著相关（ < 0.05）。Kaplan-Meier 分析显示，AKT1 和 PLK1 高表达患者的生存时间明显短于低表达患者（ < 0.05）。Spearman 秩相关检验显示，OSCC 组织中 AKT1 和 PLK1 的表达呈强相关性（ = 0.53； < 0.05）。GEPIA 生物信息学数据库分析结果显示，AKT1 和 PLK1 的表达分析及 AKT1 和 PLK1 的相关性分析与实验结果一致。
结论：AKT1 和 PLK1 的表达与 OSCC 的发生和进展有关，可能作为 OSCC 的诊断和预后指标。OSCC 组织中 AKT1 和 PLK1 之间可能存在相关性。
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