Vittal Kavitha, Pandian Sathasivasubramanian Sankara, Joseph Leena Dennis, Raj Shilpa Germaine
Department of Oral Medicine and Radiology, Bangalore Institute of Dental Sciences, Bengaluru, Karnataka, India.
Department of Oral Medicine and Radiology, Sri Ramachandra Dental College and Hospital, Sri Ramachandra University, Chennai, Tamil Nadu, India.
Indian J Dent Res. 2018 Mar-Apr;29(2):171-175. doi: 10.4103/ijdr.IJDR_59_17.
Polo-like kinase 1 (PLK1) is a critical molecule in the proliferation of several human cancers. Overexpression of PLK1 has been correlated with cancer cell proliferation and lower overall survival rates. Although PLK1 has been studied in various tumors, information regarding its expression in oral cancer and precancer is limited. Aims: This study is aimed at evaluating the expression of PLK1 in a potentially malignant and malignant disorder of the oral cavity, namely, oral submucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC), respectively, using the immunohistochemistry technique. It also intended to evaluate the association of the various histological grades of OSCC with the intensity of PLK1 expression.
Thirty OSMF, thirty OSCC tissues, and thirty control tissues were obtained, and the expression of PLK1 was detected by immunohistochemistry using rabbit antihuman PLK1 polyclonal antibodies (Abcam Ab47867). The association between staining intensity and histological grade of OSCC was evaluated.
Using SPSS 20 version, a test for proportions, nonparametric Chi-square/correlation analysis was used to compare differences in proportions of categorical variables of interest between groups. Results: PLK1 was positively expressed in 27 (90%) OSCC tissues. OSMF showed no detectable staining in 27 (90%) tissues and positive staining in 3 (10%) tissues. PLK1 showed no staining (0%) in normal tissues. Statistically significant associations were not found between staining intensity and histological grade of OSCC.
PLK1 could be a promising progression marker for OSCC. Therapeutically, targeting PLK1 may be a new approach to fight oral cancer.
polo样激酶1（PLK1）是几种人类癌症增殖中的关键分子。PLK1的过表达与癌细胞增殖及较低的总生存率相关。尽管PLK1已在多种肿瘤中得到研究，但关于其在口腔癌及癌前病变中的表达信息有限。目的：本研究旨在分别使用免疫组织化学技术评估PLK1在口腔潜在恶性和恶性疾病，即口腔黏膜下纤维化（OSMF）和口腔鳞状细胞癌（OSCC）中的表达。本研究还旨在评估OSCC不同组织学分级与PLK1表达强度之间的关联。
获取30份OSMF组织、30份OSCC组织及30份对照组织，使用兔抗人PLK1多克隆抗体（Abcam Ab47867）通过免疫组织化学检测PLK1的表达。评估染色强度与OSCC组织学分级之间的关联。
使用SPSS 20版本，采用比例检验、非参数卡方检验/相关性分析比较各感兴趣组间分类变量比例的差异。结果：27份（90%）OSCC组织中PLK1呈阳性表达。27份（90%）OSMF组织未检测到染色，3份（10%）组织呈阳性染色。正常组织中PLK1无染色（0%）。未发现OSCC染色强度与组织学分级之间存在统计学显著关联。
PLK1可能是OSCC一个有前景的进展标志物。在治疗方面，靶向PLK1可能是对抗口腔癌的一种新方法。
