Luo Bi, Lv Jia, Li Kejie, Liao Peiran, Chen Peng
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.
Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials, Guangzhou, China.
Front Nutr. 2022 Jun 9;9:916976. doi: 10.3389/fnut.2022.916976. eCollection 2022.
This study aimed to extract polysaccharides from L. var. (finger citron fruits) and analyze their structures and potential bioactivities. A new polysaccharide named K-CMLP was isolated and purified by Diethylaminoethylcellulose (DEAE)-Sepharose Fast Flow and DEAE-52 cellulose column chromatography with an average molecular weight of 3.76 × 10 kDa. Monosaccharide composition analysis revealed that K-CLMP consisted of rhamnose, galactose, and glucose, with a molar ratio of 6.75:5.87:1.00. Co-resolved by methylation and two-dimensional nuclear magnetic resonance (NMR), K-CLMP was alternately connected with 1, 2-Rha and 1, 4-Gal to form the backbone, and a small number of glucose residues was connected to O-4 of rhamnose. The results of and radical scavenging assays indicated that both crude polysaccharide Citrus medica L. var. polysaccharide (CMLP) and K-CLMP exhibited strong free-radical-scavenging properties in a dose-dependent manner. In addition, K-CMLP significantly inhibited the production of pro-inflammatory cytokines (IL-6 and TNF-α) and reactive oxygen species (ROS) in RAW 264.7 cells treated with LPS. These results provide a basis for further use as one of the potential functions of food or natural medicine.
本研究旨在从佛手柑果实中提取多糖，并分析其结构和潜在生物活性。通过二乙氨基乙基纤维素（DEAE）-琼脂糖快速流动柱和DEAE-52纤维素柱色谱法分离纯化出一种名为K-CMLP的新多糖，其平均分子量为3.76×10 kDa。单糖组成分析表明，K-CLMP由鼠李糖、半乳糖和葡萄糖组成，摩尔比为6.75:5.87:1.00。通过甲基化和二维核磁共振（NMR）共同解析，K-CLMP由1,2-鼠李糖和1,4-半乳糖交替连接形成主链，少量葡萄糖残基连接到鼠李糖的O-4位。抗氧化和自由基清除试验结果表明，粗多糖佛手柑多糖（CMLP）和K-CLMP均表现出较强的自由基清除能力，且呈剂量依赖性。此外，K-CMLP显著抑制了用脂多糖处理的RAW 264.7细胞中促炎细胞因子（IL-6和TNF-α）和活性氧（ROS）的产生。这些结果为其作为食品或天然药物的潜在功能之一的进一步应用提供了依据。
