Chen Haiqiang, Wang Jing, Liu Xiaojuan, Zhou Aimei, Xiao Jie, Huang Kaixin, Chen Hanmin, Cao Yong
Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods College of Food Science South China Agricultural University Guangzhou China.
Guangdong Zhancui Food co., Ltd. Chaozhou China.
Food Sci Nutr. 2020 Feb 18;8(3):1636-1648. doi: 10.1002/fsn3.1450. eCollection 2020 Mar.
The development of antiaging functional products is a hot topic in the field of functional foods. However, the efficient extraction of functional ingredients is the limiting step for the functional food industry. Continuous phase-transition extraction (CPE) is a new extraction technique that combines the advantages of Soxhlet extraction and supercritical extraction, which may have a distinct advantage over traditional methods in the extraction of flavonoids. In our study, the Box-Behnken design combined with response surface methodology was used to optimize CPE of crude flavonoids from finger citron fruit. The antiaging activities of finger citron crude flavonoids (FCCF) were evaluated by () model. The optimal extraction conditions for CPE were as follows: ethanol concentration 85%, temperature 90°C, time 120 min, and pressure 0.2 MPa. Compared with the heat reflux extraction, the extraction rate and content of FCCF extracted by CPE increased by 24.28% and 33.22% ( < .05), respectively. FCCF extended the lifespan of by 14.94% without causing adverse effects on their reproduction and locomotion ability. A further analysis suggested that FCCF prolonged the lifespan of nematodes under normal and oxidative stress by increasing the activity of major enzymes in endogenous antioxidant defense system and reducing the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA). The results confirmed the effectiveness of CPE in extracting crude flavonoids from finger citron fruit, and the extracted FCCF exhibited strong antiaging activities.
抗衰老功能产品的开发是功能食品领域的一个热门话题。然而，功能成分的高效提取是功能食品行业的限制步骤。连续相转变萃取（CPE）是一种结合了索氏提取和超临界萃取优点的新型萃取技术，在黄酮类化合物的萃取方面可能比传统方法具有明显优势。在我们的研究中，采用Box-Behnken设计结合响应面法对佛手果实中粗黄酮的CPE进行优化。通过（）模型评估佛手粗黄酮（FCCF）的抗衰老活性。CPE的最佳萃取条件如下：乙醇浓度85%、温度90℃、时间120分钟、压力0.2兆帕。与热回流萃取相比，CPE萃取的FCCF萃取率和含量分别提高了24.28%和33.22%（P<0.05）。FCCF使（）的寿命延长了14.94%，且对其繁殖和运动能力没有产生不利影响。进一步分析表明，FCCF通过提高内源性抗氧化防御系统中主要酶的活性，减少活性氧（ROS）和丙二醛（MDA）的积累，从而延长了正常和氧化应激条件下线虫的寿命。结果证实了CPE在从佛手果实中提取粗黄酮方面的有效性，且提取的FCCF表现出较强的抗衰老活性。
