Bai Meijuan, Cao Peijuan, Lin Yijun, Yu Pengcheng, Song Shuo, Chen Lingling, Wang Lan, Chen Yan
CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
Front Nutr. 2022 Jun 9;9:892435. doi: 10.3389/fnut.2022.892435. eCollection 2022.
Dietary restriction has a profound effect in altering immune system and promoting metabolic health and aging. However, how dietary restriction impacts erythroid system is largely unknown. We found that a short-term caloric restriction (CR) stimulates expression of KLF1, a master regulator of erythroid development, in the spleen of mouse, and thus explored the potential effect of CR on erythropoiesis.
We analyzed the effects of intermittent CR and continuous CR for different lengths of time on parameters of peripheral blood and erythroid profiles in the spleen and bone marrow in C57BL/6 mice. We next assessed how different types of CR affect phenylhydrazine-induced anemia in the mice. Colony formation assay was also used to analyze LK + progenitors and BFU-E in the bone marrow.
Intermittent CR for 2 weeks raised the number of reticulocytes in the blood, while continuous CR for 2 weeks elevated red blood cells and hemoglobin level. Intermittent CR for 2 weeks promoted extramedullary hematopoiesis in the spleen, while continuous CR mainly promoted erythropoiesis in the bone marrow. Interestingly, a short-term intermittent CR but not continuous CR was able to ameliorate phenylhydrazine-induced anemia. Intermittent CR reduced early-stage erythroblasts and increased late-stage erythroblasts/mature RBCs in the spleen, indicating an accelerated transition from early-stage to late-stage erythroblasts/mature red blood cells. Furthermore, a short-term intermittent CR elevated LK + progenitors and the committed erythroid progenitor cells BFU-E in the bone marrow.
Our study demonstrated that a short-term intermittent CR, but not continuous CR, has a significant effect to promote hematopoiesis and such activity can ameliorate phenylhydrazine-induced acute anemia in the mouse.
饮食限制对改变免疫系统、促进代谢健康和衰老具有深远影响。然而，饮食限制如何影响红系系统在很大程度上尚不清楚。我们发现短期热量限制（CR）可刺激小鼠脾脏中红系发育的主要调节因子KLF1的表达，因此探索了CR对红细胞生成的潜在影响。
我们分析了间歇性CR和不同时长的持续性CR对C57BL/6小鼠外周血参数以及脾脏和骨髓中红系特征的影响。接下来，我们评估了不同类型的CR如何影响小鼠苯肼诱导的贫血。集落形成试验也用于分析骨髓中的LK+祖细胞和BFU-E。
间歇性CR 2周可提高血液中网织红细胞数量，而持续性CR 2周可提高红细胞和血红蛋白水平。间歇性CR 2周可促进脾脏中的髓外造血，而持续性CR主要促进骨髓中的红细胞生成。有趣的是，短期间歇性CR而非持续性CR能够改善苯肼诱导的贫血。间歇性CR减少了脾脏中早期成红细胞，并增加了晚期成红细胞/成熟红细胞，表明从早期成红细胞到晚期成红细胞/成熟红细胞的转变加速。此外，短期间歇性CR可提高骨髓中LK+祖细胞和定向红系祖细胞BFU-E的水平。
我们的研究表明，短期间歇性CR而非持续性CR对促进造血具有显著作用，且这种活性可改善小鼠苯肼诱导的急性贫血。
