Albedewi Hadeel, Bindayel Iman, Albarrag Ahmed, Banjar Hanaa
Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
Department of Pathology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Front Nutr. 2022 Jun 9;9:884104. doi: 10.3389/fnut.2022.884104. eCollection 2022.
Children with cystic fibrosis (CF) are expected to have suboptimal serum vitamin D status and altered gut microbiota. The altered gut microbiota is hypothesized to have a pro-inflammatory effect that further complicates the existing respiratory inflammation. Emerging evidence suggests an association between vitamin D and gut microbiota. The aim of this study was to assess the relationships between 25-hydroxyvitamin D [25(OH)D] status, pulmonary function, and fecal bacteria in children with CF.
In this cross-sectional study, a total of 35 children with CF (8.7 ± 2.83 years) and 24 controls without CF (9 ± 2.7 years) were included in this study. Serum 25(OH)D status was measured using the Elecsys vitamin D total II assay. In the CF group, gut microbiota composition was assessed using real-time PCR analysis. Pulmonary function tests (PFTs) were measured using spirometry. Comparisons between the CF and non-CF controls were conducted using the independent sample -test. In the CF group, one-way analysis of variance (ANOVA) was used to assess differences in PFTs and gut microbiota composition across the three vitamin D subgroups. The correlations between 25(OH)D status and PFTs, or gut microbiota composition, and PFTs with gut microbiota composition were analyzed using the Pearson's correlation coefficient test.
Children with CF had significantly lower serum 25(OH)D levels compared with children without CF (44.3 ± 22.4 vs. 59 ± 25.5, respectively, = 0.026). Children with CF with optimal serum 25(OH)D level had significantly higher levels of , and total bacteria ( = 0.007, = 0.007, and = 0.022, respectively). The level of was found to be significantly higher in mild forced expiratory volume in 1 s (FEV1) compared with moderate FEV1 ( = 0.032), whereas the level of the other bacteria species was comparable across FEV1 severity groups.
Our findings may encourage studies that target and modify gut microbiota to potentially achieve better outcomes in terms of respiratory function in CF.
囊性纤维化（CF）患儿的血清维生素D水平预计不理想，且肠道微生物群会发生改变。据推测，肠道微生物群的改变具有促炎作用，会使现有的呼吸道炎症进一步复杂化。新出现的证据表明维生素D与肠道微生物群之间存在关联。本研究的目的是评估CF患儿的25-羟维生素D[25(OH)D]水平、肺功能和粪便细菌之间的关系。
在这项横断面研究中，共纳入了35名CF患儿（8.7±2.83岁）和24名无CF的对照儿童（9±2.7岁）。使用电化学发光免疫分析法检测血清25(OH)D水平。在CF组中，使用实时PCR分析评估肠道微生物群组成。使用肺活量测定法进行肺功能测试（PFT）。CF组与非CF对照组之间的比较采用独立样本t检验。在CF组中，使用单因素方差分析（ANOVA）评估三个维生素D亚组之间PFT和肠道微生物群组成的差异。使用Pearson相关系数检验分析25(OH)D水平与PFT之间、或肠道微生物群组成与PFT之间的相关性。
与无CF的儿童相比，CF患儿的血清25(OH)D水平显著更低（分别为44.3±22.4和59±25.5，P=0.026）。血清25(OH)D水平最佳的CF患儿的双歧杆菌、嗜酸乳杆菌和总细菌水平显著更高（分别为P=0.007、P=0.007和P=0.022）。发现轻度1秒用力呼气量（FEV1）时的双歧杆菌水平显著高于中度FEV1时（P=0.032），而其他细菌种类的水平在FEV1严重程度组之间相当。
我们的研究结果可能会鼓励开展针对并改变肠道微生物群的研究，以期在CF的呼吸功能方面取得更好的结果。
