Department of Endocrinology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
The Key Laboratory of Laboratory Medical Diagnostics in the Ministry of Education and Department of Clinical Biochemistry, College of Laboratory Medicine, Chongqing Medical University, Chongqing, China.
Front Endocrinol (Lausanne). 2022 Jun 9;13:893142. doi: 10.3389/fendo.2022.893142. eCollection 2022.
Animal studies have found that GPHB5 has a similar effect on system metabolism as TSH. However, the relationship between GPHB5 and metabolic diseases remains unknown. This study investigates the relationship between GPHB5 and MetS in young women.
Bioinformatics analysis was undertaken to explore the relationship between GPHB5 and metabolic-related genes and signaling pathways. EHC and OGTT were performed on all individuals. Lipid-infusion, physical activity, and cold-exposure tests were performed on healthy individuals. Serum GPHB5 concentrations were measured by an ELISA kit.
PPI network showed that 11 genes interacted with GPHB5, in which POMC and KISS1R were involved in glucose and lipid metabolism. GO analysis showed 56 pathways for BP and 16 pathways for MF, in which OPRM1 and MCR families were related to energy metabolism. KEGG analysis found that GPHB5 is associated with lipolysis and neuroactive ligand-receptor interaction pathways. The levels of circulating GPHB5 were significantly increased, while serum adiponectin levels were lower in MetS women compared with healthy women. Obese/overweight individuals had lower adiponectin levels and higher GPHB5 levels. Circulating GPHB5 levels were positively correlated with BMI, WHR, blood pressure, FBG, 2 h-BG, HbA1c, FIns, 2h-Ins, LDL-C, FFA, HOMA-IR, and AUCg, etc. but negatively correlated with HDL-C, adiponectin, and M-values. Serum GPHB5 levels did not change significantly during the OGTT, EHC, and lipid infusion. Physical activity and cold-exposure tests did not lead to changes in GPHB5 levels. GLP-1RA treatment resulted in a significant decrease in serum GPHB5 levels.
GPHB5 may be a biomarker for MetS.
动物研究发现，GPHB5 对系统代谢的影响与 TSH 相似。然而，GPHB5 与代谢疾病之间的关系尚不清楚。本研究调查了 GPHB5 与年轻女性代谢综合征（MetS）之间的关系。
通过生物信息学分析，探讨 GPHB5 与代谢相关基因和信号通路的关系。对所有个体进行 EHC 和 OGTT 检查。对健康个体进行脂质输注、体力活动和冷暴露试验。采用 ELISA 试剂盒测定血清 GPHB5 浓度。
PPI 网络显示，有 11 个基因与 GPHB5 相互作用，其中 POMC 和 KISS1R 参与葡萄糖和脂质代谢。GO 分析显示，BP 有 56 条通路，MF 有 16 条通路，其中 OPRM1 和 MCR 家族与能量代谢有关。KEGG 分析发现，GPHB5 与脂肪分解和神经活性配体-受体相互作用途径有关。与健康女性相比，MetS 女性的循环 GPHB5 水平显著升高，而血清脂联素水平较低。肥胖/超重个体的脂联素水平较低，GPHB5 水平较高。循环 GPHB5 水平与 BMI、WHR、血压、FBG、2h-BG、HbA1c、FIns、2h-Ins、LDL-C、FFA、HOMA-IR 和 AUCg 等呈正相关，与 HDL-C、脂联素和 M 值呈负相关。OGTT、EHC 和脂质输注期间，血清 GPHB5 水平无明显变化。体力活动和冷暴露试验未导致 GPHB5 水平发生变化。GLP-1RA 治疗可显著降低血清 GPHB5 水平。
GPHB5 可能是 MetS 的生物标志物。
