Reproductive Medicine Center, Yue Bei People's Hospital, Shantou University Medical College, Shaoguan, China.
Medical Research Center, Yue Bei People's Hospital, Shantou University Medical College, Shaoguan, China.
Front Immunol. 2022 Jun 10;13:845585. doi: 10.3389/fimmu.2022.845585. eCollection 2022.
The is a main activator of ATPase of Hsp90. Hsp90 is involved in various metabolic and developmental processes of tumor cells. Although, the role of in tumor cells is still unrecognized. In the current research, the RNA-seq of 33 tumors were downloaded using The Cancer Genome Atlas (TCGA) database for the analysis of expression in tumors. The Kaplan-Meier method was used for the evaluation of the prognostic significance of in patients with pan-cancer. Additionally, the correlation between and immune cell infiltration, immune checkpoint, pyroptosis-related molecules, epithelial cell transformation-related molecules, and autophagy-related molecules were analyzed by co-expression. Furthermore, we examined the effect of knockdown on cell function in Huh7 and HCCLM3 cells of hepatocellular carcinoma (HCC) cell lines. According to the finding of this study, up-regulation of expression was observed in numerous tumor tissues, and its over-expression in liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), and esophageal carcinoma (ESCA) could affect the overall survival and disease-specific survival of the patients. Meanwhile, as per the correlation analysis the expression of was greatly correlated with the expression of various immune cell infiltrates, immune checkpoint inhibitors, tumor mutation load, and microsatellite instability. Moreover, this study focused on analyzing the association of expression with multiple pathological stages in HCC, and confirmed that was an independent prognostic factor of HCC by univariate and multivariate COX regression in TCGA and The International Cancer Genome Consortium (ICGC) cohorts. At the same time, cellular experiments proved that the knockdown could decrease the proliferation activity, cell migration and invasion ability of HCC cells. Therefore, the results of this study indicated that can be used as a potential prognostic biomarker of tumors and it may have a significant role in the proliferation as well as migration of HCC cells.
该 是 Hsp90 的 ATPase 的主要激活剂。Hsp90 参与肿瘤细胞的各种代谢和发育过程。尽管如此，该 在肿瘤细胞中的作用仍未被识别。在目前的研究中，通过使用癌症基因组图谱 (TCGA) 数据库下载了 33 个肿瘤的 RNA-seq，以分析肿瘤中 的表达。Kaplan-Meier 方法用于评估 在泛癌患者中的预后意义。此外，通过共表达分析了 与免疫细胞浸润、免疫检查点、焦亡相关分子、上皮细胞转化相关分子和自噬相关分子的相关性。此外，我们还研究了敲低 在肝癌细胞系 Huh7 和 HCCLM3 细胞中的细胞功能的影响。根据本研究的结果，观察到许多肿瘤组织中 的表达上调，其在肝肝细胞癌 (LIHC)、肺腺癌 (LUAD) 和食管癌 (ESCA) 中的过表达会影响患者的总生存率和疾病特异性生存率。同时，根据相关性分析， 的表达与各种免疫细胞浸润、免疫检查点抑制剂、肿瘤突变负荷和微卫星不稳定性的表达密切相关。此外，本研究重点分析了 在 HCC 多个病理分期中的表达与多种病理分期的相关性，并通过 TCGA 和国际癌症基因组联盟 (ICGC) 队列的单因素和多因素 COX 回归证实， 在 HCC 中是独立的预后因素。同时，细胞实验证明，敲低 可以降低 HCC 细胞的增殖活性、细胞迁移和侵袭能力。因此，本研究的结果表明， 可以作为肿瘤的潜在预后生物标志物，并且可能在 HCC 细胞的增殖和迁移中发挥重要作用。
