Lion Country Safari, Loxahatchee, FL 33470, USA.
White Oak Conservation, Yulee, FL 32097, USA.
J Zoo Wildl Med. 2022 Jun;53(2):319-330. doi: 10.1638/2021-0072.
Capillary zone electrophoresis (CZE) and an immunoassay for serum amyloid A (SAA) were used to examine serum samples from clinically normal and abnormal southern white rhinoceros () and southern black rhinoceros () under managed care. CZE resolved seven fractions as well as subfractions for α1 globulins. Reference intervals were calculated for white rhinoceros ( = 33) and found to have some differences over previously reported intervals generated using agarose gel electrophoresis (AGE) methods in sera from free-ranging animals. In addition, the coefficient of variation related to fraction quantitation was found to be overlapping or superior to that reported for AGE. No significant differences were observed in CZE measurands and total protein between clinically normal and abnormal rhinoceros. In contrast to CZE, significant differences in SAA levels ( < 0.001) were observed in samples from the white rhinoceros between clinically normal and abnormal animals. In addition, in limited sample sets with repeated measures, SAA provided prognostic value. Future studies should generate more robust reference intervals and delineate the application of both SAA quantitation and CZE in routine health assessments and in prognostication.
毛细管区带电泳（CZE）和血清淀粉样蛋白 A（SAA）免疫测定法用于检查在管理下的临床正常和异常南部白犀牛（）和南部黑犀牛（）的血清样本。CZE 解析了七个分数以及α1 球蛋白的亚分数。为白犀牛（= 33）计算了参考区间，并且发现与使用琼脂糖凝胶电泳（AGE）方法在来自自由放养动物的血清中生成的先前报道的区间有一些差异。此外，与分数量化相关的变异系数被发现与报告的 AGE 重叠或优于 AGE。在临床正常和异常犀牛之间，CZE 测量值和总蛋白没有观察到显著差异。与 CZE 相反，在白犀牛的临床正常和异常动物之间的样本中观察到 SAA 水平（<0.001）的显著差异。此外，在具有重复测量的有限样本集中，SAA 提供了预后价值。未来的研究应生成更强大的参考区间，并阐明 SAA 定量和 CZE 在常规健康评估和预后中的应用。
