Osorio Shirley, Soto Jeny, Schmitt Dennis, Kiso Wendy, Cray Carolyn
Division of Comparative Pathology, Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL, United States.
White Oak Conservation, Yulee, FL, United States.
Front Vet Sci. 2023 Sep 7;10:1204880. doi: 10.3389/fvets.2023.1204880. eCollection 2023.
Serum protein electrophoresis has been demonstrated to have utility in diagnostic workup, wellness exams, and prognosis. Agarose gel electrophoresis (AGE) has previously been described for use with serum from Asian elephants (). As the newer method of capillary zone electrophoresis (CZE) is becoming more commonplace in veterinary diagnostic laboratories, serum samples from Asian elephants were examined using this method. CZE allowed for a reproducible definition of two beta fractions and, overall, showed a low coefficient of variation for fraction quantitation. Preliminary reference intervals were generated using samples primarily from an older population of 22 female elephants. Albumin levels determined by CZE were also compared with those determined by the bromocresol green method on a chemistry analyzer. It was found that the latter method overestimated the level of albumin with a mean positive bias of 11.6% or 0.38 g/dL, thus method-specific reference intervals should be used. Significant negative correlations were observed between A/G ratio determined by CZE and serum amyloid A levels (r = -0.47, < 0.0001) and haptoglobin ( = -0.52, < 0.0001); both APP were significantly correlated with the alpha 2 globulin fraction (p < 0.0001). CZE reflects an overall picture of changes in acute phase proteins and immunoglobulins and accurate quantitation of albumin and thus should be considered as an adjunct tool to the use of other measures of the acute phase response in patient monitoring.
血清蛋白电泳已被证明在诊断检查、健康体检和预后评估中具有实用价值。琼脂糖凝胶电泳（AGE）此前已被描述用于亚洲象血清检测（）。随着毛细管区带电泳（CZE）这种更新的方法在兽医诊断实验室中越来越普遍，本研究使用该方法检测了亚洲象的血清样本。CZE能够对两个β组分进行可重复的定义，总体而言，组分定量的变异系数较低。初步参考区间主要使用来自22头老年雌性大象群体的样本生成。还将CZE测定的白蛋白水平与化学分析仪上溴甲酚绿法测定的白蛋白水平进行了比较。结果发现，后一种方法高估了白蛋白水平，平均正偏差为11.6%或0.38 g/dL，因此应使用特定方法的参考区间。观察到CZE测定的A/G比值与血清淀粉样蛋白A水平（r = -0.47，< 0.0001）和触珠蛋白（ = -0.52，< 0.0001）之间存在显著负相关；两种急性时相蛋白均与α2球蛋白组分显著相关（p < 0.0001）。CZE反映了急性期蛋白和免疫球蛋白变化的总体情况，并能准确测定白蛋白，因此在患者监测中应被视为急性期反应其他检测方法的辅助工具。
