Department of Veterinary Services, Zoo Atlanta, Atlanta, GA 30315, USA,
Department of Veterinary Services, Zoo Atlanta, Atlanta, GA 30315, USA.
J Zoo Wildl Med. 2022 Jun;53(2):442-447. doi: 10.1638/2020-0211.
Electrophoresis is a practical diagnostic tool for the identification of changes in serum protein fractions, which can be associated with a variety of diseases. Protein electrophoresis studies in Ursidae are limited, and currently no published fraction values are available for the giant panda (). The aim of this study was to describe the serum protein fractions in the giant panda using both capillary zone electrophoresis (CZE) and standard agarose gel electrophoresis (AGE) techniques. Serum samples from nine healthy giant pandas (n = 19) were used for this study. Samples were evaluated using CZE and standard AGE. The CZE procedure successfully resolved serum proteins into seven fractions: prealbumin; albumin; and α1-, α2-, β1-, β2-, and γ-globulin; while AGE separated serum into only six protein fractions: prealbumin; albumin; α1-, α2-, and β-globulins; and γ-globulin. These data will serve as a preliminary baseline for further studies and provide insight for the medical management of giant pandas.
电泳是鉴定血清蛋白组分变化的实用诊断工具,这些变化可能与多种疾病有关。熊科动物的蛋白电泳研究有限,目前尚无大熊猫()的已发表的蛋白组分值。本研究旨在使用毛细管区带电泳(CZE)和琼脂糖凝胶电泳(AGE)技术描述大熊猫的血清蛋白组分。本研究使用了 9 只健康大熊猫(n=19)的血清样本。使用 CZE 和标准 AGE 对样本进行评估。CZE 程序成功地将血清蛋白分为 7 个组分:前白蛋白;白蛋白;以及α1-、α2-、β1-、β2-和γ-球蛋白;而 AGE 则将血清分离为仅 6 种蛋白组分:前白蛋白;白蛋白;α1-、α2-和β-球蛋白;以及γ-球蛋白。这些数据将作为进一步研究的初步基准,并为大熊猫的医疗管理提供参考。