Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Division of Comparative Pathology, Department of Pathology & Laboratory Medicine, University of Miami Miller School of Medicine, Miami, Florida, USA.
Vet Clin Pathol. 2023 Sep;52(3):452-460. doi: 10.1111/vcp.13210. Epub 2023 Mar 27.
Electrophoresis can be used to aid in the diagnosis of different diseases in avian species. Capillary zone electrophoresis (CZE) is an automated method that is proposed to be superior to the dye methods used in agarose gel electrophoresis (AGE). However, reference intervals (RIs) for CZE in avian species and comparison studies between electrophoretic methods are lacking.
The goals of the current study were to compare AGE and CZE methods and determine reference intervals for CZE using plasma from bald eagles (Haliaeetus leucocephalus).
Heparinized plasma samples from 44 bald eagles (mean age 18.7 years) under managed care were examined by AGE and CZE. Method comparison analyses were completed, as well as the generation of preliminary RIs using the CZE method and ASVCP guidelines.
Passing-Bablok regression and Bland-Altman plots demonstrate that these methods are not equivalent. All fractions were significantly correlated between the methods except for alpha 1 globulin. Inter-assay and intra-assay CVs for CZE were lower or comparable to AGE and ranged from 2.4% to 15.4%, and 0.8% to 8.3%, respectively. CZE resolved more fractions than AGE with two fractions observed in the beta and gamma region vs one for AGE in each region.
CZE provided improved resolution and reproducibility for the quantitation of protein fractions in the bald eagle. Although most fraction results correlated with AGE, these methods were judged as not equivalent, necessitating method-specific Rls. Reference intervals generated from a limited number of mostly aged individuals under managed care should be considered preliminary; additional studies will aid in the production of more robust intervals.
电泳可用于辅助诊断禽类的不同疾病。毛细管区带电泳（CZE）是一种自动化方法，据提议优于琼脂糖凝胶电泳（AGE）中使用的染料方法。然而，禽类 CZE 的参考区间（RI）和电泳方法之间的比较研究尚缺乏。
本研究的目的是比较 AGE 和 CZE 方法，并确定使用白头海雕（Haliaeetus leucocephalus）血浆的 CZE 参考区间。
对 44 只接受管理护理的白头海雕（平均年龄 18.7 岁）的肝素化血浆样本进行 AGE 和 CZE 检查。完成了方法比较分析，并根据 CZE 方法和 ASVCP 指南生成初步 RI。
Passing-Bablok 回归和 Bland-Altman 图表明这些方法不等效。除了α1球蛋白外，两种方法之间所有分数均显著相关。CZE 的批内和批间 CV 低于或可与 AGE 相媲美，范围分别为 2.4%至 15.4%和 0.8%至 8.3%。CZE 比 AGE 解析出更多的分数，在β和γ区观察到两个分数，而 AGE 在每个区都观察到一个分数。
CZE 为白头海雕中蛋白质分数的定量提供了更好的分辨率和重现性。尽管大多数分数结果与 AGE 相关，但这些方法被判断为不等效，需要特定方法的 RI。从数量有限的、主要是在管理护理下的个体中生成的参考区间应被视为初步的；更多的研究将有助于产生更稳健的区间。
