大熊猫（Ailuropoda melanoleuca）乳汁样本的成分及寡糖
Composition and oligosaccharides of a milk sample of the giant panda, Ailuropoda melanoleuca.
作者信息
Nakamura Tadashi, Urashima Tadasu, Mizukami Taiji, Fukushima Michihiro, Arai Ikichi, Senshu Tatsudo, Imazu Koji, Nakao Tatsuko, Saito Tadao, Ye Zhiyong, Zuo Hong, Wu Kongju
机构信息
Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan.
出版信息
Comp Biochem Physiol B Biochem Mol Biol. 2003 Jul;135(3):439-48. doi: 10.1016/s1096-4959(03)00093-9.
A milk sample from a captive giant panda (Ailuropoda melanoleuca), obtained at 13 days postpartum, contained 7.1% protein, 1.6% carbohydrate, 10.4% lipid and 0.9% ash. The ratio of casein to whey proteins was 5.0:2.1. Sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) of the whey protein fraction showed the presence of at least two major proteins other than alpha-lactalbumin and beta-lactoglobulin. SDS-PAGE and urea-gel electrophoresis showed that alphas-casein is not a major component. The proportions of triacylglycerol, cholesterol, cholesterol esters and phospholipid were 90.5, 5.3, 0.96 and 3.1%, of the total lipid, respectively. The dominant saccharide in the panda milk was Gal(alpha1-3)Gal(beta1-4)Glc (isoglobotriose). The milk contained, in addition, lesser amounts of lactose, Gal(alpha1-3)Gal(beta1-4)[Fuc(alpha1-3)]Glc (fucosyl isoglobotriose), Neu5Ac(alpha2-3)Gal(beta1-4)Glc (3'-N-acetylneuraminyl-lactose), Neu5Ac(alpha2-6)Gal(beta1-4)Glc (6'-N-acetylneuraminyl-lactose) and Neu5Ac(alpha2-3)Gal(beta1-4)[Fuc(alpha1-3)]Glc.
一份产后13天采集的圈养大熊猫（大熊猫）乳汁样本，蛋白质含量为7.1%，碳水化合物含量为1.6%，脂质含量为10.4%，灰分含量为0.9%。酪蛋白与乳清蛋白的比例为5.0:2.1。乳清蛋白组分的十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）显示，除α-乳白蛋白和β-乳球蛋白外，至少还存在两种主要蛋白质。SDS-PAGE和尿素凝胶电泳表明，αs-酪蛋白不是主要成分。三酰甘油、胆固醇、胆固醇酯和磷脂分别占总脂质的90.5%、5.3%、0.96%和3.1%。大熊猫乳汁中的主要糖类是Gal(α1-3)Gal(β1-4)Glc（异球三糖）。此外，乳汁中还含有少量乳糖、Gal(α1-3)Gal(β1-4)[Fuc(α1-3)]Glc（岩藻糖基异球三糖）、Neu5Ac(α2-3)Gal(β1-4)Glc（3'-N-乙酰神经氨酸乳糖）、Neu5Ac(α2-6)Gal(β1-4)Glc（6'-N-乙酰神经氨酸乳糖）和Neu5Ac(α2-3)Gal(β1-4)[Fuc(α1-3)]Glc。
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